LOCUS       Exported                 571 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-Gal4DBD-GBP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     35..54
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             125..565
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
       61 catcattttg gcaaagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtgc
      121 caccatgaag ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct
      181 caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg
      241 ctactctccc aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc
      301 aaggctagaa agactggaac agctatttct actgattttt cctcgagaag accttgacat
      361 gattttgaaa atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca
      421 agataatgtg aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc
      481 tctaacattg agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa
      541 aggtcaaaga cagttgactg tatcggctag c
//
