LOCUS       Exported                 641 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of 
            dtomato and luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pUltra-Chili-Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 641)
  TITLE     pUltra series
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(45..101)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(194..217)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(392..411)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatac tagaaggccc gggattctcc
       61 tccacgtcac ctgcttgttt gagtagtgag aagtttgttg ctccagatcc cttgtacagc
      121 tcgtccatgc cgtacaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg
      181 atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg
      241 ggcagttgca cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg
      301 ccgtccttca gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac
      361 aggcgctcgg tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg
      421 gggaagttgg tgccgcgcat cttcaccttg tagatcagcg tgccgtcctg cagggaggag
      481 tcctgggtca cggtcaccag accgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      541 ccctcgggga aggacagctt cttgtaatcg gggatgtcgg cggggtgctt cacgtacgcc
      601 ttggagccgt acatgaactg gggggacagg atgtcccagg c
//
