LOCUS Exported 10227 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION ReaChR-citrine in lentival transfer vector under human synapsin promoter. ACCESSION . VERSION . KEYWORDS pLenti-ReaChR-citrine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10227) AUTHORS Lin JY, Knutsen PM, Muller A, Kleinfeld D, Tsien RY TITLE ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. JOURNAL Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1. PUBMED 23995068 REFERENCE 2 (bases 1 to 10227) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10227 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 239..618 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 620..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 773..793 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 836..1016 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1063..1188 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1681..1914 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2099..2143 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2441..2558 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2633..3080 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" CDS 4166..4882 /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4211..4232) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4472..4491) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4819..4840 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4919..5507 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4972..4992) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5390..5401) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5510..5526) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5511..5527) /label=pBluescriptKS /note="For pBluescript vector" LTR 6032..6212 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6238..6255) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6244..6468 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6514..6942 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6601..6620) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6811..6832 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6951..6971) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6956..7285 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7136..7271 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7198..7217 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7333..7380 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7399..7773 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7903..8024 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7940..7959) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7994..8013 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8073..8089) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8073..8089) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8086..8108) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8097..8113 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8121..8151) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8166..8187 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8304..8321) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8475..9063) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8555..8574) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9234..10094) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9857..9876 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10095..10199) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgttta tgcgctgctt cgcgatgtac gggccagata tacgcgttga 241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc 841 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 901 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 961 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 1021 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 1081 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 1141 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 1201 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 1261 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 1321 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 1381 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 1441 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 1501 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 1561 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1621 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1681 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1741 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1801 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1861 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1921 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1981 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 2041 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 2101 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 2161 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 2221 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 2281 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 2341 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg 2401 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 2461 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 2521 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 2581 gagatccagt ttggttaatt aagtgtctag actgcagagg gccctgcgta tgagtgcaag 2641 tgggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc 2701 cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga 2761 ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc 2821 cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt 2881 cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg 2941 ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc 3001 tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg 3061 tgtcgtgcct gagagcgcag tcgagaaggt accggatcca ccggtgacca tggtgagcag 3121 aagaccctgg ctgctggccc tggccctggc cgtggccctg gccgccggca gcgccggcgc 3181 cagcaccggc agcgacgcca ccgtgcccgt ggccacccag gacggccccg actacgtgtt 3241 ccacagagcc cacgagagaa tgctgttcca gaccagctac accctggaga acaacggcag 3301 cgtgatctgc atccccaaca acggccagtg cttctgcctg gcctggctga agagtaacgg 3361 caccaacgcc gagaagctgg ccgccaacat cctgcagtgg gtggtgtttg cgctgagcgt 3421 ggcgtgcctg ggctggtatg cgtatcaggc gtggcgcgcg acctgcggct gggaagaagt 3481 gtatgtggcg ctgattgaaa tgatgaaaag cattattgaa gcgtttcatg aatttgatag 3541 cccggcgacc ctgtggctga gcagcggcaa cggcgtggtg tggatgcgct atggcgaatg 3601 gctgctgacc tgccccgtga ttctgattca tctgagcaac ctgaccggcc tgaaagatga 3661 ttatagcaaa cgcaccatgg gcctgctggt gagcgacgtg ggctgcattg tgtggggcgc 3721 gaccagcgcg atgtgcaccg gctggaccaa aattctgttt tttctgatta gcctgagcta 3781 tggcatgtat acctattttc atgcggccaa agtgtatatt gaagcgtttc ataccgtgcc 3841 gaaaggcctg tgcagacagc tggtgagagc catggcctgg ctgttcttcg tgagctgggg 3901 catgttcccc gtgctgttcc tgctgggccc cgagggcttc ggccatatta gcccgtatgg 3961 cagcgcgatt ggccatagca ttctggatct gattgcgaag aacatgtggg gcgtgctggg 4021 caactatctg cgcgtgaaaa ttcatgaaca tattctgctg tatggcgata ttcgcaaaaa 4081 acagaaaatt accattgcgg gccaggaaat ggaagtggaa accctggtgg cggaagaaga 4141 agataagtac gagagcagcc tcgagatggt gagcaagggc gaggagctgt tcaccggggt 4201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 4261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 4321 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt 4381 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 4441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 4501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 4561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 4621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 4681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 4741 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc 4801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 4861 cggcatggac gagctgtaca agtaggctag ccccgaattc gatatcaagc ttatcgataa 4921 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 4981 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5041 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5101 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5161 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5221 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5281 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 5341 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5401 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5461 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg 5521 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 5581 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca 5641 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 5701 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg 5761 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat 5821 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt 5881 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat 5941 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca 6001 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc 6061 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6121 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6181 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct 6241 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 6301 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 6361 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 6421 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 6481 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 6541 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 6601 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 6661 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 6721 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 6781 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 6841 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 6901 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 6961 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 7021 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 7081 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 7141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 7201 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 7261 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 7321 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac 7381 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc 7441 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg 7501 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag 7561 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg 7621 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg 7681 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac 7741 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc 7801 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 7861 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 7921 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 7981 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 8041 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 8101 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 8161 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 8221 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 8281 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 8341 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 8401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 8461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 8521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 8581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 8641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 8701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 8761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 8881 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 8941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 9001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 9061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 9121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 9181 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 9241 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 9301 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 9361 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 9421 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 9481 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 9541 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 9601 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 9661 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 9721 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9781 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9841 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 9901 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 9961 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 10021 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 10081 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 10141 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 10201 acatttcccc gaaaagtgcc acctgac //