LOCUS Exported 969 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance. ACCESSION . VERSION . KEYWORDS pBUN501 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 969) AUTHORS Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ TITLE A CRISPR/Cas9 toolkit for multiplex genome editing in plants. JOURNAL BMC Plant Biol. 2014 Nov 29;14(1):327. PUBMED 25432517 REFERENCE 2 (bases 1 to 969) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..969 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(311..331) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(338..403) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" ORIGIN 1 aagaatcgtc aaccttcgcc atctcgttgg agaaaatctc ctgcaggtag cagatgcgat 61 tcttcctgcg cgtgtaccgc ctgcgggcgg tgcgcttgag ccgcgtagcc tcagccgtct 121 cgccgctgtc gaacaggaga gcgccaatga gattcttctt gatggaatgc cgatcggtgt 181 tgcccaggac cttgaacttc tttgagggca ccttgtactc gtcggtgatc acggcccagc 241 caacagagtt agtcccaatg gcgaggccga tcgagtactt cttgtcagca gctggcaccc 301 cgtggatgcc aaccttcctc ttcttcttcg gagccatctt gtcatcatca tccttgtaat 361 caatgtcgtg gtccttgtaa tccccgtcgt ggtccttgta atccatctag agcggccgcg 421 gccatacaag tattggggat cccccgggct gcagaagtaa caccaaacaa cagggtgagc 481 atcgacaaaa gaaacagtac caagcaaata aatagcgtat gaaggcaggg ctaaaaaaat 541 ccacatatag ctgctgcata tgccatcatc caagtatatc aagatcaaaa taattataaa 601 acatacttgt ttattataat agataggtac tcaaggttag agcatatgaa tagatgctgc 661 atatgccatc atgtatatgc atcagtaaaa cccacatcaa catgtatacc tatcctagat 721 cgatatttcc atccatctta aactcgtaac tatgaagatg tatgacacac acatacagtt 781 ccaaaattaa taaatacacc aggtagtttg aaacagtatt ctactccgat ctagaacgaa 841 tgaacgaccg cccaaccaca ccacatcatc acaaccaagc gaacaaaaag catctctgta 901 tatgcatcag taaaacccgc atcaacatgt atacctatcc tagatcgata tttccatcca 961 tcatcttca //