LOCUS Exported 11177 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system). ACCESSION . VERSION . KEYWORDS pSLQ1658-dCas9-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11177) AUTHORS Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B TITLE Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. JOURNAL Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. PUBMED 24360272 REFERENCE 2 (bases 1 to 11177) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11177 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 579..920 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 987..1403 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1333..1355 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1371..1387 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" regulatory 1417..1426 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1432..1452 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1453..5553 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5563..5583 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5593..6309 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 5593..6306 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(5635..5656) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5896..5915) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6243..6264 /label=EGFP-C /note="EGFP, forward primer" promoter 6320..6819 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6355..6378) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6752..6771 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6840..7439 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6840..6859) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7336..7356 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7539..8053 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" primer_bind complement(8222..8238) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8222..8238) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8235..8257) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8246..8262 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8270..8300) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8315..8336 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8453..8470) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8624..9212) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8704..8723) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9383..10243) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10006..10025 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10244..10348) /gene="bla" /label=AmpR promoter primer_bind 10416..10434 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(10472..10494) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 10594..10613 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10807..10829 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 10957..10976 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" LTR 11176..515 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" ORIGIN 1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1381 ccctcactcc ttctctaggc gccggaatta gatctcgcca ccatggctag ccccaaaaag 1441 aagaggaaag tggacaagaa gtattctatc ggactggcca tcgggactaa tagcgtcggg 1501 tgggccgtga tcactgacga gtacaaggtg ccctctaaga agttcaaggt gctcgggaac 1561 accgaccggc attccatcaa gaaaaatctg atcggagctc tcctctttga ttcaggggag 1621 accgctgaag caacccgcct caagcggact gctagacggc ggtacaccag gaggaagaac 1681 cggatttgtt accttcaaga gatattctcc aacgaaatgg caaaggtcga cgacagcttc 1741 ttccataggc tggaagaatc attcctcgtg gaagaggata agaagcatga acggcatccc 1801 atcttcggta atatcgtcga cgaggtggcc tatcacgaga aatacccaac catctaccat 1861 cttcgcaaaa agctggtgga ctcaaccgac aaggcagacc tccggcttat ctacctggcc 1921 ctggcccaca tgatcaagtt cagaggccac ttcctgatcg agggcgacct caatcctgac 1981 aatagcgatg tggataaact gttcatccag ctggtgcaga cttacaacca gctctttgaa 2041 gagaacccca tcaatgcaag cggagtcgat gccaaggcca ttctgtcagc ccggctgtca 2101 aagagccgca gacttgagaa tcttatcgct cagctgccgg gtgaaaagaa aaatggactg 2161 ttcgggaacc tgattgctct ttcacttggg ctgactccca atttcaagtc taatttcgac 2221 ctggcagagg atgccaagct gcaactgtcc aaggacacct atgatgacga tctcgacaac 2281 ctcctggccc agatcggtga ccaatacgcc gaccttttcc ttgctgctaa gaatctttct 2341 gacgccatcc tgctgtctga cattctccgc gtgaacactg aaatcaccaa ggcccctctt 2401 tcagcttcaa tgattaagcg gtatgatgag caccaccagg acctgaccct gcttaaggca 2461 ctcgtccggc agcagcttcc ggagaagtac aaggaaatct tctttgacca gtcaaagaat 2521 ggatacgccg gctacatcga cggaggtgcc tcccaagagg aattttataa gtttatcaaa 2581 cctatccttg agaagatgga cggcaccgaa gagctcctcg tgaaactgaa tcgggaggat 2641 ctgctgcgga agcagcgcac tttcgacaat gggagcattc cccaccagat ccatcttggg 2701 gagcttcacg ccatccttcg gcgccaagag gacttctacc cctttcttaa ggacaacagg 2761 gagaagattg agaaaattct cactttccgc atcccctact acgtgggacc cctcgccaga 2821 ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag aaactatcac tccctggaac 2881 ttcgaagagg tggtggacaa gggagccagc gctcagtcat tcatcgaacg gatgactaac 2941 ttcgataaga acctccccaa tgagaaggtc ctgccgaaac attccctgct ctacgagtac 3001 tttaccgtgt acaacgagct gaccaaggtg aaatatgtca ccgaagggat gaggaagccc 3061 gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa gaccaataga 3121 aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg cttcgactct 3181 gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta ccatgatctg 3241 cttaagatca tcaaggacaa ggattttctg gacaatgagg agaacgagga catccttgag 3301 gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga gaggcttaag 3361 acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag aagatatacc 3421 ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca gagcggaaag 3481 accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat gcaacttatc 3541 catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc tggacaaggt 3601 gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa gaagggtatt 3661 ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca taaaccagag 3721 aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca gaagaacagc 3781 agggagcgga tgaaaagaat tgaggaaggg attaaggagc tcgggtcaca gatccttaaa 3841 gagcacccgg tggaaaacac ccagcttcag aatgagaagc tctatctgta ctaccttcaa 3901 aatggacgcg atatgtatgt ggaccaagag cttgatatca acaggctctc agactacgac 3961 gtggacgcca tcgtccctca gagcttcctc aaagacgact caattgacaa taaggtgctg 4021 actcgctcag acaagaaccg gggaaagtca gataacgtgc cctcagagga agtcgtgaaa 4081 aagatgaaga actattggcg ccagcttctg aacgcaaagc tgatcactca gcggaagttc 4141 gacaatctca ctaaggctga gaggggcgga ctgagcgaac tggacaaagc aggattcatt 4201 aaacggcaac ttgtggagac tcggcagatt actaaacatg tcgcccaaat ccttgactca 4261 cgcatgaata ccaagtacga cgaaaacgac aaacttatcc gcgaggtgaa ggtgattacc 4321 ctgaagtcca agctggtcag cgatttcaga aaggactttc aattctacaa agtgcgggag 4381 atcaataact atcatcatgc tcatgacgca tatctgaatg ccgtggtggg aaccgccctg 4441 atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg gagactacaa ggtctacgac 4501 gtgcgcaaga tgattgccaa atctgagcag gagatcggaa aggccaccgc aaagtacttc 4561 ttctacagca acatcatgaa tttcttcaag accgaaatca cccttgcaaa cggtgagatc 4621 cggaagaggc cgctcatcga gactaatggg gagactggcg aaatcgtgtg ggacaagggc 4681 agagatttcg ctaccgtgcg caaagtgctt tctatgcctc aagtgaacat cgtgaagaaa 4741 accgaggtgc aaaccggagg cttttctaag gaatcaatcc tccccaagcg caactccgac 4801 aagctcattg caaggaagaa ggattgggac cctaagaagt acggcggatt cgattcacca 4861 actgtggctt attctgtcct ggtcgtggct aaggtggaaa aaggaaagtc taagaagctc 4921 aagagcgtga aggaactgct gggtatcacc attatggagc gcagctcctt cgagaagaac 4981 ccaattgact ttctcgaagc caaaggttac aaggaagtca agaaggacct tatcatcaag 5041 ctcccaaagt atagcctgtt cgaactggag aatgggcgga agcggatgct cgcctccgct 5101 ggcgaacttc agaagggtaa tgagctggct ctcccctcca agtacgtgaa tttcctctac 5161 cttgcaagcc attacgagaa gctgaagggg agccccgagg acaacgagca aaagcaactg 5221 tttgtggagc agcataagca ttatctggac gagatcattg agcagatttc cgagttttct 5281 aaacgcgtca ttctcgctga tgccaacctc gataaagtcc ttagcgcata caataagcac 5341 agagacaaac caattcggga gcaggctgag aatatcatcc acctgttcac cctcaccaat 5401 cttggtgccc ctgccgcatt caagtacttc gacaccacca tcgaccggaa acgctatacc 5461 tccaccaaag aagtgctgga cgccaccctc atccaccaga gcatcaccgg actttacgaa 5521 actcggattg acctctcaca gctcggaggg gatgagggag ctcccaagaa aaagcgcaag 5581 gtaggtagtt ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 5641 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 5701 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 5761 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 5821 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 5881 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 5941 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 6001 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 6061 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 6121 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 6181 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 6241 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 6301 tacaagtaag aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc 6361 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat 6421 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct 6481 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg 6541 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa 6601 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg 6661 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg 6721 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct 6781 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca 6841 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 6901 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 6961 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 7021 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 7081 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 7141 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 7201 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 7261 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 7321 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 7381 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac 7441 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa 7501 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt 7561 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat 7621 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata 7681 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg 7741 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg 7801 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc 7861 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct 7921 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc 7981 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc 8041 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag 8101 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac 8161 tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat tcgtaatcat 8221 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 8281 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 8341 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 8401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 8461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 8521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 8581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 8641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 8761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 8821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 8881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 8941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9061 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 9181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 9241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 9301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 9361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 9421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 9481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 9541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 9601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 9781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 9841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 9901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 10021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 10081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 10141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 10201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 10261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 10321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 10381 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 10441 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10501 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 10561 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 10621 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 10681 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 10741 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 10801 ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat 10861 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct 10921 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc 10981 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt 11041 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata 11101 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct 11161 ccagaaaaag gggggaa //