LOCUS Exported 7865 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS integrin alpha9 EGFP-N3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7865) TITLE Other integrin alpha9 plasmids REFERENCE 2 (bases 1 to 7865) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7865 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 3805..3814 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3811..4530 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3811..4527 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3856..3877) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4117..4136) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4464..4485 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 4653..4774 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4690..4709) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4744..4763 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4781..5236) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4918..4939) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5130..5149 /label=F1ori-R /note="F1 origin, reverse primer" promoter 5263..5367 /gene="bla" /label=AmpR promoter promoter 5369..5726 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(5392..5412) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5577..5712 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5639..5658 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5761..6555 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5815..5834) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6425..6444 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6743..6762) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6787..6834 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 7163..7751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7652..7671 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttaga gcgggcgcta tggggagccg gacgccagag 661 tcccctctcc atgccgtgca gctgcgctgg ggcccccggc gccgaccccc gctgctgccg 721 ctgctattgc tgctcgtgcc gccgccaccg cgggtcgggg gctacaacct ggatccgcag 781 cgccccgtgc acttccaagg ccccgctgac tcgttcttcg gctacgcagt tctggagcat 841 ttccacgaca acacgcgctg ggtccttgtg ggcgcaccaa aggcagattc caaatacagc 901 ccttcagtga agtctcctgg ggctgtgttt aagtgccgtg ttcacaccaa ccctgaccgg 961 agatgcaccg aactggacat ggctcgaggg aagaatcggg gcacgtcctg cggaaagacc 1021 tgccgggaag accgcgatga tgagtggatg ggggtgagcc tggcccgaca gcccaaggct 1081 gatggccgtg tgttggcctg tgctcatcgc tggaagaaca tctactatga agccgaccac 1141 atcctacccc atggcttctg ctacatcatc ccctccaacc tccaggccaa aggcaggacg 1201 ctgatccctt gctatgaaga gtataagaag aagtacggag aggaacacgg ctcctgccag 1261 gctgggatag cgggcttctt caccgaggag ctggtggtga tgggtgctcc agggtcattt 1321 tattgggctg gaaccatcaa agtgctgaac cttacggaca acacctattt aaaactgaac 1381 gacgaagtga tcatgaacag gcggtacacc tacctgggct acgcagtgac cgctggccac 1441 ttctctcacc cgtccaccat tgatgtggta ggaggtgccc cacaggacaa aggcatcggc 1501 aaggtttata ttttcagagc tgaccgaaga tcaggcacct taattaagat ctttcaagca 1561 tcaggtaaaa agatgggctc ttacttcggc tcctccttgt gcgcagttga cctgaatggg 1621 gacggcctct ctgacctgct ggtgggggcc cccatgtttt ctgagatcag ggatgaggga 1681 caggtcactg tctacatcaa cagaggaaat ggagccctcg aggagcagct ggctctgact 1741 ggggatggtg cctacaatgc ccactttgga gagagcattg ccagcctgga cgatctggac 1801 aatgatgggt tcccagatgt ggccattggt gcacccaagg aggatgactt cgcaggggcg 1861 gtctatatct atcatggtga tgccggtggg atagtccctc agtactcaat gaaactgtct 1921 gggcagaaga taaatccagt gctccggatg tttggtcagt ccatatcggg aggcattgat 1981 atggatggaa atggctatcc tgatgtcact gttggagcct tcatgtccga cagcgtggtt 2041 cttctcagag caaggcctgt cattacggtg gatgtctcca tcttcctccc gggctccatc 2101 aacatcacag cgcctcagtg tcacgacgga cagcagcctg tgaactgcct gaacgtcacc 2161 acctgcttca gcttccatgg caaacacgtt ccagaagaga ttggcctgaa ttatgttctg 2221 atggctgacg tggccaaaaa ggagaagggc cagatgccca gggtctactt tgtgctgctg 2281 ggagagacca tgggtcaggt cacagagaag ctgcagctga cttacatgga ggagacgtgt 2341 cgtcactatg tggcccatgt gaagcggagg gtgcaggacg tcatcagccc gatcgtgttt 2401 gaagcagcct acagcctcag tgagcatgtg actggagagg aggagaggga actgccgcct 2461 ctgacaccag ttctccgctg gaaaaaggga caaaagattg cccaaaagaa tcagactgtt 2521 tttgaaagga attgccgttc agaggactgt gccgcagacc tgcagcttca gggtaaactg 2581 ctgctctcca gtatggatga gaaaaccctg tatctagctt tgggggctgt gaagaacatc 2641 tccctaaaca tctctatctc caacctcgga gatgatgcct atgatgccaa cgtgtccttc 2701 aatgtttccc gggagctctt cttcatcaac atgtggcaga aggaggagat gggcatctcc 2761 tgtgagctgc tggaatcgga cttcctcaaa tgcagcgtgg gatttccttt catgaggtca 2821 aagtcaaagt atgaattcag cgtgatcttt gatacaagcc acctgtctgg ggaagaggaa 2881 gttctcagct tcattgttac tgctcagagt ggcaacacgg agcgctctga atccctgcat 2941 gacaacaccc tcgtgctgat ggtgccactg atgcacgagg tggacacgtc catcaccgga 3001 atcatgtctc caacctcctt tgtatatggc gagtccgtgg acgcagccaa cttcattcag 3061 ctggatgacc tggagtgtca ctttcagccc atcaatatca cccttcaggt ctacaacact 3121 ggcccaagca cccttccagg gtcatctgtc agcatctctt tccctaatcg actctcatct 3181 ggtggtgcag agatgtttca tgtccaggaa atggtggtgg gccaagagaa gggaaactgc 3241 tctttccaga aaaacccaac tccctgcatc atccctcaag aacaagaaaa tatcttccac 3301 acaatatttg cttttttcac aaagtctgga agaaaagtct tggactgtga aaaaccagga 3361 atttcttgcc taacagcaca ctgtaacttt agtgctcttg ctaaagaaga aagtcgtact 3421 atagacattt acatgctgct gaacacagaa atactgaaaa aggacagttc gtctgtcatc 3481 cagttcatgt cccgcgccaa ggtgaaggtg gatcctgccc taagggtggt ggaaatagct 3541 catgggaacc cagaagaggt gacggtggtc ttcgaggccc tgcacaatct ggagccccgt 3601 ggctacgtcg tggggtggat catcgccatc agtttgttgg tgggaatcct catcttcctg 3661 ctgctggccg tactagtctg gaagatgggc ttctttcgcc gaaggtacaa agaaattatc 3721 gaagctgaga agaaccggaa agagaatgaa gacagttggg actgggtcca gaaaaaccag 3781 ggtaccgcgg gcccgggatc catcgccacc atggtgagca agggcgagga gctgttcacc 3841 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 3901 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 3961 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 4021 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 4081 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 4141 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 4201 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 4261 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 4321 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 4381 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 4441 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 4501 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc 4561 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 4621 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 4681 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4741 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt 4801 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 4861 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 4921 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 4981 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 5041 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 5101 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 5161 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 5221 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 5281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 5341 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 5401 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 5461 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 5521 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 5581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5701 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 5761 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 5821 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 5881 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 5941 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 6001 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 6061 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 6121 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 6181 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 6241 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 6301 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 6361 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 6421 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 6481 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 6541 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 6601 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 6661 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 6721 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 6781 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 6841 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 6901 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 6961 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 7021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 7081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 7141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 7201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 7261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 7321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 7381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 7441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 7501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 7561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 7621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 7681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 7741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 7801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 7861 tgcat //