LOCUS Exported 6566 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA3-CD14 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6566) TITLE TLR Signaling Plasmids REFERENCE 2 (bases 1 to 6566) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6566 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 938..947 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(2118..2136) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2119..2136) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2156..2173) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2162..2386 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2432..2860 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2519..2538) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2729..2750 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2869..2889) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2874..3204 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3055..3190 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3117..3136 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3271..4065 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3325..3344) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3935..3954 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4239..4360 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4276..4295) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4330..4349 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4409..4425) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4409..4425) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4422..4444) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4433..4449 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4457..4487) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4502..4523 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4640..4657) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4811..5399) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4891..4910) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5570..6430) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6193..6212 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6431..6535) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggcc accatggagc gcgcgtcctg 961 cttgttgctg ctgctgctgc cgctggtgca cgtctctgcg accacgccag aaccttgtga 1021 gctggacgat gaagatttcc gctgcgtctg caacttctcc gaacctcagc ccgactggtc 1081 cgaagccttc cagtgtgtgt ctgcagtaga ggtggagatc catgccggcg gtctcaacct 1141 agagccgttt ctaaagcgcg tcgatgcgga cgccgacccg cggcagtatg ctgacacggt 1201 caaggctctc cgcgtgcggc ggctcacagt gggagccgca caggttcctg ctcagctact 1261 ggtaggcgcc ctgcgtgtgc tagcgtactc ccgcctcaag gaactgacgc tcgaggacct 1321 aaagataacc ggcaccatgc ctccgctgcc tctggaagcc acaggacttg cactttccag 1381 cttgcgccta cgcaacgtgt cgtgggcgac agggcgttct tggctcgccg agctgcagca 1441 gtggctcaag ccaggcctca aggtactgag cattgcccaa gcacactcgc ctgccttttc 1501 ctgcgaacag gttcgcgcct tcccggccct taccagccta gacctgtctg acaatcctgg 1561 actgggcgaa cgcggactga tggcggctct ctgtccccac aagttcccgg ccatccagaa 1621 tctagcgctg cgcaacacag gaatggagac gcccacaggc gtgtgcgccg cactggcggc 1681 ggcaggtgtg cagccccaca gcctagacct cagccacaac tcgctgcgcg ccaccgtaaa 1741 ccctagcgct ccgagatgca tgtggtccag cgccctgaac tccctcaatc tgtcgttcgc 1801 tgggctggaa caggtgccta aaggactgcc agccaagctc agagtgctcg atctcagctg 1861 caacagactg aacagggcgc cgcagcctga cgagctgccc gaggtggata acctgacact 1921 ggacgggaat cccttcctgg tccctggaac tgccctcccc cacgagggct caatgaactc 1981 cggcgtggtc ccagcctgtg cacgttcgac cctgtcggtg ggggtgtcgg gaaccctggt 2041 gctgctccaa ggggcccggg gctttgccta agatccaaga caggcggccg ctcgagcatg 2101 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg 2161 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2221 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2281 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2341 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2401 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2581 aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 2641 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 2701 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 2761 aaccctatct cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg 2821 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 2881 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat 2941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3121 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 3181 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 3241 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 3301 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 3361 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3421 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 3481 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3541 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3601 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3661 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3721 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3781 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 3841 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3901 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 3961 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 4021 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 4081 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 4141 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 4201 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 4261 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 4321 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 4381 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 4441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 4501 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 4561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 4621 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 4681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 4741 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 4801 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 4861 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 4921 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 4981 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 5041 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 5101 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 5161 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 5221 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 5281 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 5341 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 5401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 5461 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 5521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 5581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 5641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 5701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 5761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 5821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 5881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 5941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 6001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 6061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 6121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 6181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 6241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 6301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 6361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 6421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 6481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 6541 ttccccgaaa agtgccacct gacgtc //