LOCUS       Exported               11495 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 
            promoter for genome editing. SV40 NLS is fused to the C terminal. 
            GAL4 region from pACT2 is removed..
ACCESSION   .
VERSION     .
KEYWORDS    pACT2-CAS9c
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 11495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             131..991
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1936..2076
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2002..2021)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2162..2184
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2218..2614
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2618..6721
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6734..6754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6890..7077
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6945..6969)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    7095..7128
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    7200..7233
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    7277..7310
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(7928..9022)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9035..9439)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      9916..11258
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10879..10926)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     1981 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     2041 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     2101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa
     2221 cttcttttct ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg
     2281 acaaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     2341 atgtcgttgt tccagagctg atgaggggta tcttcgaaca cacgaaactt tttccttcct
     2401 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     2461 aatttgaaat aaaaaaagtt tgccgctttg ctatcaagta taaatagacc tgcaattatt
     2521 aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac
     2581 aatatttcaa gctataccaa gcatacaatc aactccaatg gacaagaagt actccattgg
     2641 gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc
     2701 gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat
     2761 tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc
     2821 acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa
     2881 tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga
     2941 ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta
     3001 ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa
     3061 ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt
     3121 cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact
     3181 ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc
     3241 caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca
     3301 gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct
     3361 gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa
     3421 agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga
     3481 cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt
     3541 gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca
     3601 ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa
     3661 ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag
     3721 ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga
     3781 gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg
     3841 aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga
     3901 tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat
     3961 accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa
     4021 atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc
     4081 ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct
     4141 tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa
     4201 atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat
     4261 cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta
     4321 tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa
     4381 cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga
     4441 caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga
     4501 tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat
     4561 gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa
     4621 tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt
     4681 tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat
     4741 ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc
     4801 aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt
     4861 caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca
     4921 aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat
     4981 aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa
     5041 tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact
     5101 ggacatcaat cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa
     5161 agatgattct attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga
     5221 taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa
     5281 cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct
     5341 gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac
     5401 caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa
     5461 actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa
     5521 ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta
     5581 cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt
     5641 tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga
     5701 aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac
     5761 cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga
     5821 aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc
     5881 catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga
     5941 aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc
     6001 caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa
     6061 agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat
     6121 catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa
     6181 agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa
     6241 cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact
     6301 gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc
     6361 tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga
     6421 gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga
     6481 taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa
     6541 cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga
     6601 caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat
     6661 tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga
     6721 cagcagggct gaccccaaga agaagaggaa ggtgtgattg gacttcttcg ccagaggttt
     6781 ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt acgaaaagat
     6841 ggaaaagggt caaatcgttg gtagatacgt tgttgacact tctaaataag cgaatttctt
     6901 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     6961 aagtgactct taggttttaa aacgaaaatt cttgttcttg agtaactctt tcctgtaggt
     7021 caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat
     7081 gtgcgaggga cctaataact tcgtatagca tacattatac gaagttatat taagggttcc
     7141 ggatcgcggc cgctcgacct gcagccaagc tagcggacgt aaactcctct tcagacctaa
     7201 taacttcgta tagcatacat tatacgaagt tatattaagg gttattgaat atgatcggaa
     7261 ttggtcgagg gacctaataa cttcgtatag catacattat acgaagttat attaagggtt
     7321 ccggatcgcg gccgctcgac ctgcagccag gctagcttgg ctggacgtaa actcctcttc
     7381 agacctaata acttcgatag catacattat acgaagttat attaagggtt attgatatga
     7441 tcggaattgg tgcactacgt gcttaaggcc gtttctgaca gagtaaaatt cttgagggaa
     7501 ctttcaccat tatgggaaat ggttcaagaa ggtattgact taaactccat caaatggtca
     7561 ggtcattgag tgttttttat ttgttgtatt tttttttttt tagagaaaat cctccaatat
     7621 caaattagga atcgtagttt catgattttc tgttacacct aactttttgt gtggtgccct
     7681 cctccttgtc aatattaatg ttaaagtgca attctttttc cttatcacgt tgagccatta
     7741 gtatcaattt gcttacctgt attcctttac tatcctcctt tttctccttc ttgataaatg
     7801 tatgtagatt gcgtatatag tttcgtctac cctatgaaca tattccattt tgtaatttcg
     7861 tgtcgtttct attatgaatt tcatttataa agtttatgta caaatatcat aaaaaaagag
     7921 aatcttttta agcaaggatt ttcttaactt cttcggcgac agcatcaccg acttcggtgg
     7981 tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc tttttaactg
     8041 catcttcaat ggccttacct tcttcaggca agttcaatga caatttcaac atcattgcag
     8101 cagacaagat agtggcgata gggtcaacct tattctttgg caaatctgga gcagaaccgt
     8161 ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc aaggacgcag
     8221 atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg atatcaccaa
     8281 acatgttgct ggtgattata ataccattta ggtgggttgg gttcttaact aggatcatgg
     8341 cggcagaatc aatcaattga tgttgaacct tcaatgtagg aaattcgttc ttgatggttt
     8401 cctccacagt ttttctccat aatcttgaag aggccaaaac attagcttta tccaaggacc
     8461 aaataggcaa tggtggctca tgttgtaggg ccatgaaagc ggccattctt gtgattcttt
     8521 gcacttctgg aacggtgtat tgttcactat cccaagcgac accatcacca tcgtcttcct
     8581 ttctcttacc aaagtaaata cctcccacta attctctgac aacaacgaag tcagtacctt
     8641 tagcaaattg tggcttgatt ggagataagt ctaaaagaga gtcggatgca aagttacatg
     8701 gtcttaagtt ggcgtacaat tgaagttctt tacggatttt tagtaaacct tgttcaggtc
     8761 taacactacc ggtaccccat ttaggaccac ccacagcacc taacaaaacg gcatcaacct
     8821 tcttggaggc ttccagcgcc tcatctggaa gtgggacacc tgtagcatcg atagcagcac
     8881 caccaattaa atgattttcg aaatcgaact tgacattgga acgaacatca gaaatagctt
     8941 taagaacctt aatggcttcg gctgtgattt cttgaccaac gtggtcacct ggcaaaacga
     9001 cgatcttctt aggggcagac ataggggcag acattagaat ggtatatcct tgaaatatat
     9061 atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga ttgctaacca
     9121 cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta ttgtgatgca
     9181 agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg cctctcacct
     9241 ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat
     9301 taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc
     9361 gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata
     9421 cctgagtatt cccacagttc cttaccactc ttttgttact ctattgatcc agctcagcaa
     9481 aggcagtgtg atctaagatt ctatcttcgc gatgtagtaa aactagctag accgagaaag
     9541 agactagaaa tgcaaaaggc acttctacaa tggctgccat cattattatc cgatgtgacg
     9601 ctgcagcttc tcaatgatat tcgaatacgc tttgaggaga tacagcctaa tatccgacaa
     9661 actgttttac agatttacga tcgtacttgt tacccatcat tgaattttga acatccgaac
     9721 ctgggagttt tccctgaaac agatagtata tttgaacctg tataataata tatagtctag
     9781 cgctttacgg aagacaatgt atgtatttcg gttcctggag aaactattgc atctattgca
     9841 taggtaatct tgcacgtcgc atccccggtt cattttctgc gtttccatct tgcacttcaa
     9901 tagcatatct ttgttaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     9961 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
    10021 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
    10081 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
    10141 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
    10201 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
    10261 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
    10321 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
    10381 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
    10441 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
    10501 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
    10561 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
    10621 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
    10681 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
    10741 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
    10801 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
    10861 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
    10921 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
    10981 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
    11041 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
    11101 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
    11161 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
    11221 tcatccttca atgctatcat ttcctttgat attggatcat atgcatagta ccgagaaact
    11281 agtgcgaagt agtgatcagg tattgctgtt atctgatgag tatacgttgt cctggccacg
    11341 gcagaagcac gcttatcgct ccaatttccc acaacattag tcaactccgt taggcccttc
    11401 attgaaagaa atgaggtcat caaatgtctt ccaatgtgag attttgggcc attttttata
    11461 gcaaagattg aataaggcgc atttttcttc aaagc
//
