LOCUS Exported 7296 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pGL-FLKif3B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7296) AUTHORS Ginkel LM, Wordeman L TITLE Expression and partial characterization of kinesin-related proteins in differentiating and adult skeletal muscle. JOURNAL Mol Biol Cell. 2000 Dec . 11(12):4143-58. PUBMED 11102514 REFERENCE 2 (bases 1 to 7296) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7296 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 385..519 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(422..441) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 476..495 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 645..1100 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(732..751) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 942..963 /label=F1ori-F /note="F1 origin, forward primer" CDS 1388..2248 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1606..1625) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" protein_bind complement(2350..2383) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." rep_origin 2461..3049 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2950..2969 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3203..3220 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3551..3567 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3556..3578 /label=M13/pUC Reverse /note="In lacZ gene" enhancer 3597..3900 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3901..4104 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4055..4075 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4101..4125 /label=LNCX /note="Human CMV promoter, forward primer" promoter 4220..4238 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 4220..4237 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 4962..4982 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" ORIGIN 1 ctctagcttg ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa 61 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt 121 aaactagctg catatgcttg ctgcttgaga gttttgctta ctgagtatga tttatgaaaa 181 tattatacac aggagctagt gattctaatt gtttgtgtat tttagattca cagtcccaag 241 gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag ccataccaca 301 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 361 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 481 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atcctgcatt aatgaatcgg 541 ccaacgcgcg gggagaggcg gtttgcgtat tggctggcgt aatagcgaag aggcccgcac 601 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 841 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 901 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 961 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 1021 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 1081 aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 1141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgccaggtc ttggactggt 1201 gagaacggct tgctcggcag cttcgatgtg tgctggaggg agaataaagg tctaagatgt 1261 gcgatagagg gaagtcgcat tgaattatgt gctgtgtagg gatcgctggt atcaaatatg 1321 tgtgcccacc cctggcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 1381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 1441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 1501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 1561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 1621 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 1681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 1741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 1801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 1861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 1921 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 1981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 2041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 2101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 2161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 2221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 2281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 2341 tctcatgcca taacttcgta taatgtatgc tatacgaagt tatggcatga ccaaaatccc 2401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 2461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 2521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 2581 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 2641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 2701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 2761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 2821 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg 2881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 2941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 3121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 3181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 3241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg 3301 cgtttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 3421 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtactac 3481 gaggcccttt cactcattag gcaccccagg ctttacactt tatagcttcc ggctcgtata 3541 atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagcatcgt gcaggtcgtt 3601 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 3661 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 3721 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 3781 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 3841 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 3901 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 3961 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 4021 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 4081 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat 4141 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcc 4201 taggcttttg caaaaagcta tttaggtgac actatagaag gtacgcctgc aggtaccggt 4261 ccggaattcc cgggtcgacg agctcactag tcggcggccg ccgccaccat gagcaagggc 4321 gaggaactgt tcactggcgt ggtcccaatt ctcgtggaac tggatggcga tgtgaatggg 4381 cacaaatttt ctgtcagcgg agagggtgaa ggtgatgcca catacggaaa gctcaccctg 4441 aaattcatct gcaccactgg aaagctccct gtgccatggc caacactggt cactaccttc 4501 acctatggcg tgcagtgctt ttccagatac ccagaccata tgaagcagca tgactttttc 4561 aagagcgcca tgcccgaggg ctatgtgcag gagagaacca tctttttcaa agatgacggg 4621 aactacaaga cccgcgctga agtcaagttc gaaggtgaca ccctggtgaa tagaatcgag 4681 ttgaagggca ttgactttaa ggaagatgga aacattctcg gccacaagct ggaatacaac 4741 tataactccc acaatgtgta catcatggcc gacaagcaaa agaatggcat caaggtcaac 4801 ttcaagatca gacacaacat tgaggatgga tccgtgcagc tggccgacca ttatcaacag 4861 aacactccaa tcggcgacgg ccctgtgctc ctcccagaca accattacct gtccacccag 4921 tctgccctgt ctaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 4981 acagctgctg ggattacaca tggcatggat gaactataca aacctaggat gtccaagtta 5041 aaaagctcag aatcagtccg ggtggtggtc cgccgtcggc ccatgaatgg caaagaaaag 5101 gctgcatctt atgacaaggt ggtagatgtg gatgtgaagc tggggcaggt gtctgtgaag 5161 aaccccaaag gcacatccca tgagatgccc aagaccttca ccttcgacgc tgtgtatgat 5221 tggaacgcca agcagtttga gctctatgat gagacgttcc gaccactcgt ggactctgtg 5281 ctgcagggtt tcaatggcac aatttttgcc tatggacaaa caggaactgg gaaaacctat 5341 accatggagg gagttcgtgg tgaccctgaa aaaagagggg tcatccccaa ctcctttgat 5401 cacatcttca cccacatctc tcgatctcag aatcagcagc acctggtccg ggcgtcttac 5461 ttagagatct atcaagaaga gattcgagac ttgctttcaa aggatcagac caaaaggcta 5521 gagctcaaag agagaccaga tacaggagtg tatgtaaagg atttgtcttc ttttgtcacg 5581 aagagtgtga aggagataga gcatgtgatg aatgtgggaa accagaaccg gtctgtcggt 5641 gctacaaaca tgaatgagca cagctcacgg tcgcatgcaa tctttgttat cactattgaa 5701 tgcagcgagg tgggcctgga tggtgaaaac cacatccggg taggaaagct gaatcttgta 5761 gatcttgctg gcagtgagcg gcaagccaag actggagctc aaggggaaag actgaaggaa 5821 gccaccaaga tcaacctgtc cctttccgcc ttgggtaatg ttatctctgc ccttgtagat 5881 ggcaaaagca cccatattcc atatagagac tcaaagctga ccaggcttct ccaagattcc 5941 cttggtggca atgccaaaac tgtgatggta gccaatgtgg ggcctgcctc ttacaatgta 6001 gaagagaccc tgaccactct gagatatgcc aaccgtgcca aaaacattaa gaacaagcca 6061 agggtcaatg aggacccaaa ggatgctctg cttcgagaat tccaagaaga aattgctcgg 6121 ctcaaggccc agctggaaaa acgttccatt ggcaggagga agaggcgaga gaagcggagg 6181 gaaggtggtg gcagtggtgg gggtggggaa gaggaggagg aggagggaga agagggtgag 6241 gaggacgggg atgataagga tgattactgg cgggaacagc aagaaaaact ggagattgag 6301 aagcgggcca ttgtagagga ccacagcttg gttgcagagg agaagatgag gctgctgaag 6361 gagaaggaga aaaagatgga ggacctgcgg cgagagaagg atgctgcaga gatgctgggt 6421 gccaaaatca aggccatgga gagtaagctg cttgtcggag gaaaaaatat agtagatcat 6481 acaaacgaac agcagaagat cctggagcaa aaacgccagg agatcgcaga gcagaaacgt 6541 cgagaaagag aaatccagca gcaaatggag agtcgagatg aggagaccct ggaactgaaa 6601 gagacttaca cctcactgca gcaggaggta gacatcaaga ccaaaaagct caaaaagctc 6661 ttttccaagc ttcaggccgt gaaggccgag atccacgacc tccaagaaga acacatcaaa 6721 gagcgccagg agctggagca gacgcagaat gaactcaccc gggagctaaa gctcaagcat 6781 cttattatag aaaactttat tcccttggaa gagaagaata aaattatgaa tagatccttt 6841 tttgatgatg aagaagacca ttggaaatta catcctataa ccagactgga aaaccagcag 6901 atgatgaagc ggcctgtctc tgctgtggga tacaagagac ctctgagcca gcatgcacgg 6961 atgtccatga tgattcggcc agagccccgg tacagggcgg agaacatcat gctcttggag 7021 ttagatatgc ccagccggac aaccagagac tacgagggcc cagccatctc tcccaaggtt 7081 caggctgcgc tggatgcagc tctgcaggat gaagatgaga tacaggtgga tgcctcatcc 7141 tttgaaagta ctgccagcag aaaacccaag gccaggccca agagtggcag gaaatcagga 7201 tcctcctctt cctcctcagg aaaccctgca tctcagtttt acccacagtc tcgggggctg 7261 gttcccaagc tcgagtaagg ggggcccgag cggccg //