LOCUS Exported 860 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION encodes yeast-optimized dCas9_VP64 synthetic transcription factor. ACCESSION . VERSION . KEYWORDS pTPGI_dCas9_VP64 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 860) AUTHORS Farzadfard F, Perli SD, Lu TK TITLE Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. JOURNAL ACS Synth Biol. 2013 Sep 11. PUBMED 23977949 REFERENCE 2 (bases 1 to 860) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..860 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(147..167) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter complement(207..648) /label=TPGI promoter /note="S. cerevisiae GAL1 promoter modified to contain two copies of the tet operator (Ellis et al., 2009)" primer_bind complement(213..236) /label=GAL1 /note="S. cerevisiae GAL1 promoter, forward primer" protein_bind 284..302 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 305..323 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 531..648 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" ORIGIN 1 ttctttttna tggagtgtcc tgatccagtg ttacccaaaa ccttgaactt cttggatgga 61 accttgtatt catcagtaat aacagcccaa ccaacagagt ttgtaccgat agccaaacca 121 atagagtatt tcttatcccc gggtctaact tttctcttct tctttggggc tctagacatt 181 cttttaatcg tggatccggg gtttttctcc ttgacgttaa agtatagagg tatattaaca 241 attttttgtt gatactttta ttacatttga ataagaagta atatctctat cactgatagg 301 gagatctcta tcactgatag ggacagtttt tgcatttata tatctgttaa tagatcaaaa 361 atcatcgctt cgctgctgca gtaccccaga aataaggcta aaaaagtaat cgcattatca 421 tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa 481 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg 541 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg 601 agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta cttcgaattc 661 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat 721 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt 781 ctctttgagc aataaagctg ataacaaaat ctttgtcgct cttcgcaacg tcaacagtac 841 ccttagtata ttctccagta //