LOCUS       Exported                 860 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  encodes yeast-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    pTPGI_dCas9_VP64
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        85..189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             374..752
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(408..427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
       61 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      841 cttctgcgct cggcccttcc
//
