LOCUS       Exported                 790 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  encodes yeast-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    pTPGI_dCas9_VP64
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        125..272
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 ctatatatat aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc
       61 agttatgacg ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc
      121 ttacgtacaa tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg
      181 aaaaaagaaa aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat
      241 acgtgattaa gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt
      301 ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag
      361 attccgatgc tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc
      421 cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc
      481 cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca
      541 atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag
      601 agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac
      661 tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg
      721 ggacaggtga acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc
      781 ccgaaagctt
//
