LOCUS Exported 6649 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION bi-directional expression vector for yeast, galactose inducible, LEU2 selection. ACCESSION . VERSION . KEYWORDS pBEVY-GL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6649) AUTHORS Miller CA 3rd, Martinat MA, Hyman LE TITLE Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae. JOURNAL Nucleic Acids Res. 1998 Aug 1;26(15):3577-83. PUBMED 9671822 REFERENCE 2 (bases 1 to 6649) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6649 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 106..127 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 142..172 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 180..196 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 185..207 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 204..220 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 204..220 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 267..454 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind 375..399 /label=pHybLex-R /note="ADH terminator, reverse primer" promoter 624..1288 /gene="GAL1 and GAL10 genes of S. cerevisiae" /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" protein_bind 840..957 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" primer_bind 1252..1275 /label=GAL1 /note="S. cerevisiae GAL1 promoter, forward primer" terminator 1325..1624 /label=ADH2 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 2 (ADH2) gene" rep_origin 1649..2995 /label=2u ori /note="yeast 2u plasmid origin of replication" CDS complement(3133..4227) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" primer_bind complement(4657..4675) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4743..4847 /gene="bla" /label=AmpR promoter CDS 4848..5708 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5066..5085) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5879..6467 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6368..6387 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6621..6638 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt 241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat 301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc 361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt 421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc 481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga 541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg 601 caggtcgact ctagaggatc ccctttcaaa aattcttact ttttttttgg atggacgcaa 661 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atacatatcc 721 atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa 781 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac 841 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc 901 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac 961 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct 1021 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt 1081 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta 1141 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg 1201 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct 1261 ctatacttta acgtcaagga gaaaaaaccc gggtaccgag ctcgaattcg acacttctaa 1321 ataagcggat ctcctatgcc ttcacgattt atagtttcca ttatcaagta tgcctatatt 1381 agtatatagc atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta 1441 ctttttgctc ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt 1501 acccattcct ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc 1561 tccaacaaac agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc 1621 tcattttcct tcgcatgccc tcgagcggaa cgaagcatct gtgcttcatt ttgtagaaca 1681 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga 1741 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta 1801 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt 1861 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt 1921 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact 1981 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc 2041 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga 2101 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa 2161 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg 2221 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc 2281 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat 2341 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta 2401 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga 2461 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt 2521 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct 2581 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat 2641 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa 2701 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt 2761 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata 2821 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc 2881 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact 2941 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcgatcc 3001 gatgataagc tgtcaaacat gagaattaat tctaccctat gaacatattc cattttgtaa 3061 tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa 3121 aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc 3181 ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt 3241 aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat 3301 tgcagcagac aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga 3361 accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga 3421 cgcagatggc aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc 3481 accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat 3541 catggcggca gaatcaatca attgatgttg aaccttcaat gtaggaaatt cgttcttgat 3601 ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa 3661 ggaccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat 3721 tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc 3781 ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt 3841 acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt 3901 acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc 3961 aggtctaaca ctacctgtac cccatttagg accacccaca gcacctaaca aaacggcatc 4021 aaccttcttg gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc 4081 agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat 4141 agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa 4201 aacgacgatc ttcttagggg cagacatagg ggcagacatt agaatgtata tccttgaaat 4261 atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaataagac gattgctaac 4321 cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg 4381 caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcctctcac 4441 ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgcggcga cctctgaaat 4501 taacaaaaaa tttccagtca tcgaatttgt ctgtgcgata gcgcccctgt gtgttctcgt 4561 tatgttgagg aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc ttacgatacc 4621 tgagtattcc cacagttaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat 4681 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 4741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 4861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 4921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 4981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 5041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 5101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 5401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 5581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 6001 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 6061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 6601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga //