LOCUS Exported 869 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter. ACCESSION . VERSION . KEYWORDS pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 869) AUTHORS Bryan Roth TITLE Roth lab DREADDs JOURNAL Unpublished REFERENCE 2 (bases 1 to 869) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..869 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(51..767) /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(93..114) /label=EGFP-C /note="EGFP, forward primer" primer_bind 442..461 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 701..722 /label=EGFP-N /note="EGFP, reverse primer" regulatory 764..773 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattctca cttgtacagc 61 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg 121 cgcttctcgt tggggtcttt gctcagtttg gactggtagc tcaggtagtg gttgtcgggc 181 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg 241 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg 301 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg 361 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc 421 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc 481 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg 541 gggtagcggg cgaagcacat caggccgtag ccgaaggtgg tcacgagggt gggccagggc 601 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg 661 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc 721 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcttatcat 781 cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac 841 ctcgactaaa cacatgtaaa gcatgtgca //