LOCUS Exported 865 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter. ACCESSION . VERSION . KEYWORDS pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 865) AUTHORS Bryan Roth TITLE Roth lab DREADDs JOURNAL Unpublished REFERENCE 2 (bases 1 to 865) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..865 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(55..771) /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(97..118) /label=EGFP-C /note="EGFP, forward primer" primer_bind 446..465 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 705..726 /label=EGFP-N /note="EGFP, reverse primer" regulatory 768..777 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcct atcacttgta 61 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg 121 atcgcgcttc tcgttggggt ctttgctcag tttggactgg tagctcaggt agtggttgtc 181 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg 241 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg 301 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag 361 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc 421 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt 481 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg 541 gtcggggtag cgggcgaagc acatcaggcc gtagccgaag gtggtcacga gggtgggcca 601 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc 661 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc 721 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggctta 781 tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt 841 taacctcgac taaacacatg taaag //