LOCUS Exported 937 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus. ACCESSION . VERSION . KEYWORDS pAW8ENdeI-cyEGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 937) AUTHORS Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, Carr AM TITLE Optimisation of the Schizosaccharomyces pombe urg1 Expression System. JOURNAL PLoS One. 2013 Dec 20;8(12):e83800. doi: 10.1371/journal.pone.0083800. PUBMED 24376751 REFERENCE 2 (bases 1 to 937) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..937 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 85..107 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 99..116 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 100..116 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(172..885) /codon_start=1 /product="green fluorescent protein" /label=GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" ORIGIN 1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat 121 tcataacttc gtatataata ccatatacga agttatcccg ggagatcttt atttgtacaa 181 ttcatccata ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc 241 tctcttttcg tttggatctt tggataaggc agattgagtg gataagtaat ggttgtctgg 301 taacaagact ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac 361 agaaccatct tcaatgttgt gtctaatttt gaagttaact ttgataccat tcttttgttt 421 gtcagccatg atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat 481 gttaccatct tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc 541 ttcaaacttg acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct 601 ttcttgaaca taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatgtgatc 661 tgggtatcta gaaaaacatt gaacaccata agttaaagta gtgactaagg ttggccatgg 721 aactggcaat ttaccagtag tacaaataaa ttttaaggtc aatttaccgt aagtagcatc 781 accttcacct tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac 841 caaaattggg acaacaccag tgaataattc ttcaccttta gacatagatc tacccatcga 901 ccccgtggag ccggttgatc ctgtagaacc agtacta //