LOCUS Exported 7329 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter. ACCESSION . VERSION . KEYWORDS hGFAP-Roxed-Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7329) AUTHORS Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P TITLE Binary recombinase systems for high-resolution conditional mutagenesis. JOURNAL Nucleic Acids Res. 2014 Jan 9. PUBMED 24413561 REFERENCE 2 (bases 1 to 7329) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7329 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2298..2318 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 2662..2796 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 2916..3050 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 3170..3304 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS 4217..4240 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" primer_bind complement(4263..4280) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4269..4493 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(4661..4678) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4832..5420) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4912..4931) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5591..6451) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6214..6233 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6452..6556) /gene="bla" /label=AmpR promoter rep_origin 6583..7038 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6670..6689) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6880..6901 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 7169..7217 /note="synthetic polyadenylation signal" misc_feature 7231..7322 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 7271..7290 /label=RVprimer3 /note="pGL3 vector, forward primer" ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgagctccca cctccctctc 61 tgtgctggga ctcacagagg gagacctcag gaggcagtct gtccatcaca tgtccaaatg 121 cagagcatac cctgggctgg gcgcagtggc gcacaactgt aattccagca ctttgggagg 181 ctgatgtgga aggatcactt gagcccagaa gttctagacc agcctgggca acatggcaag 241 accctatctc tacaaaaaaa gttaaaaaat cagccacgtg tggtgacaca cacctgtagt 301 cccagctatt caggaggctg aggtgagggg atcacttaag gctgggaggt tgaggctgca 361 gtgagtcgtg gttgcgccac tgcactccag cctgggcaac agtgagaccc tgtctcaaaa 421 gacaaaaaaa aaaaaaaaaa aaaaaagaac atatcctggt gtggagtagg ggacgctgct 481 ctgacagagg ctcgggggcc tgagctggct ctgtgagctg gggaggaggc agacagccag 541 gccttgtctg caagcagacc tggcagcatt gggctggccg ccccccaggg cctcctcttc 601 atgcccagtg aatgactcac cttggcacag acacaatgtt cggggtgggc acagtgcctg 661 cttcccgccg caccccagcc cccctcaaat gccttccgag aagcccattg agcagggggc 721 ttgcattgca ccccagcctg acagcctggc atcttgggat aaaagcagca cagcccccta 781 ggggctgccc ttgctgtgtg gcgccaccgg cggtggagaa caaggctcta ttcagcctgt 841 gcccaggaaa ggggatcagg ggatgcccag gcatggacag tgggtggcag ggggggagag 901 gagggctgtc tgcttcccag aagtccaagg acacaaatgg gtgaggggac tgggcagggt 961 tctgaccctg tgggaccaga gtggagggcg tagatggacc tgaagtctcc agggacaaca 1021 gggcccaggt ctcaggctcc tagttgggcc cagtggctcc agcgtttcca aacccatcca 1081 tccccagagg ttcttcccat ctctccaggc tgatgtgtgg gaactcgagg aaataaatct 1141 ccagtgggag acggaggggt ggccagggaa acggggcgct gcaggaataa agacgagcca 1201 gcacagccag ctcatgtgta acggctttgt ggagctgtca aggcctggtc tctgggagag 1261 aggcacaggg aggccagaca aggaaggggt gacctggagg gacagatcca ggggctaaag 1321 tcctgataag gcaagagagt gccggccccc tcttgcccta tcaggacctc cactgccaca 1381 tagaggccat gattgaccct tagacaaagg gctggtgtcc aatcccagcc cccagcccca 1441 gaactccagg gaatgaatgg gcagagagca ggaatgtggg acatctgtgt tcaagggaag 1501 gactccagga gtctgctggg aatgaggcct agtaggaaat gaggtggccc ttgagggtac 1561 agaacaggtt cattcttcgc caaattccca gcaccttgca ggcacttaca gctgagtgag 1621 ataatgcctg ggttatgaaa tcaaaaagtt ggaaagcagg tcagaggtca tctggtacag 1681 cccttccttc cctttttttt tttttttttt gtgagacaag gtctctctct gttgcccagg 1741 ctggagtggc gcaaacacag ctcactgcag cctcaaccta ctgggctcaa gcaatcctcc 1801 agcctcagcc tcccaaagtg ctgggattac aagcatgagc caccccactc agccctttcc 1861 ttccttttta attgatgcat aataattgta agtattcatc atggtccaac caaccctttc 1921 ttgacccacc ttcctagaga gagggtcctc ttgcttcagc ggtcagggcc ccagacccat 1981 ggtctggctc caggtaccac ctgcctcatg caggagttgg cgtgcccagg aagctctgcc 2041 tctgggcaca gtgacctcag tggggtgagg ggagctctcc ccatagctgg gctgcggccc 2101 aaccccaccc cctcaggcta tgccaggggg tgttgccagg ggcacccggg catcgccagt 2161 ctagcccact ccttcataaa gccctcgcat cccaggagcg agcagagcca gagcaggttg 2221 gagaggagac gcatcacctc cgctgctcgc ggggatctgc gatctaagta agcttgaatt 2281 ctcgagccgc caccatgcct aagaagaaga ggaaggtgac ctctgatgaa gtcaggaaga 2341 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc 2401 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa cgtaacttta aataattggc 2461 attatttaaa gttaatgtat gcgctacgaa gttattcgcg atgaataaat gaaagcttgc 2521 agatctgcga ctctagagga tctgcgactc tagaggatca taatcagcca taccacattt 2581 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 2641 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 2701 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 2761 tccaaactca tcaatgtatc ttatcatgtc tggatctgcg actctagagg atcataatca 2821 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 2881 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 2941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 3001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgcgactcta 3061 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 3121 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 3181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 3241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 3301 gatccccatc aagctgatcc ggaaccctta attaacttta aataattggc attatttaaa 3361 gttaaacagg aaatggttcc ctgctgaacc tgaggatgtg agggactacc tcctgtacct 3421 gcaagccaga ggcctggctg tgaagaccat ccaacagcac ctgggccagc tcaacatgct 3481 gcacaggaga tctggcctgc ctcgcccttc tgactccaat gctgtgtccc tggtgatgag 3541 gagaatcaga aaggagaatg tggatgctgg ggagagagcc aagcaggccc tggcctttga 3601 acgcactgac tttgaccaag tcagatccct gatggagaac tctgacagat gccaggacat 3661 caggaacctg gccttcctgg gcattgccta caacaccctg ctgcgcattg ccgaaattgc 3721 cagaatcaga gtgaaggaca tctcccgcac cgatggtggg agaatgctga tccacattgg 3781 caggaccaag accctggtgt ccacagctgg tgtggagaag gccctgtccc tgggggttac 3841 caagctggtg gagagatgga tctctgtgtc tggtgtggct gatgacccca acaactacct 3901 gttctgccgg gtcagaaaga atggtgtggc tgccccttct gccacctccc aactgtccac 3961 ccgggccctg gaagggatct ttgaggccac ccaccgcctg atctatggtg ccaaggatga 4021 ctctgggcag agatacctgg cctggtctgg ccactctgcc agagtgggtg ctgccaggga 4081 catggccagg gctggtgtgt ccatccctga aatcatgcag gctggtggct ggaccaatgt 4141 gaacattgtg atgaactaca tcagaaacct ggactctgag actggggcca tggtgaggct 4201 gctcgaggat ggggacgatt acaaggatga cgacgataag taggtcagag ctcgctgatc 4261 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 4321 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 4381 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 4441 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 4501 ggcggaaaga accagctggg gtcgaccgat gcccttgaga gccttcaacc cagtcagctc 4561 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 4621 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc 4681 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 4741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 4801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 4861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 4921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 4981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 5041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 5101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 5281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 5401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 5461 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 5521 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 5581 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 5641 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 5701 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 5761 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 5821 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 5881 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 5941 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6001 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 6061 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 6121 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 6181 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 6241 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 6301 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 6361 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 6421 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 6481 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 6541 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg 6601 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 6661 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 6721 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 6781 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 6841 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 6901 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 6961 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 7021 tattaacgtt tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat 7081 cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta 7141 aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg 7201 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa 7261 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct 7321 ctatcgata //