LOCUS Exported 599 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Modified pBluescript containing a telomeric insert.. ACCESSION . VERSION . KEYWORDS f.pRST1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 599) AUTHORS Arat NO, Griffith JD TITLE Human Rap1 interacts directly with telomeric DNA and regulates TRF2 localization at the telomere. JOURNAL J Biol Chem. 2012 Dec 7;287(50):41583-94. doi: 10.1074/jbc.M112.415984. Epub 2012 Oct 20. PUBMED 23086976 REFERENCE 2 (bases 1 to 599) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..599 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(174..190) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(174..190) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(227..247) /label=T3 /note="T3 promoter, forward primer" promoter complement(227..245) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(266..282) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(266..282) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(279..301) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 290..306 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(314..344) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 359..380 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(497..514) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gacggtatcg ataagcttga agacttaggg ttagggttag ggttagggtt agggttaggg 61 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg 121 ttagggttag ggttagggtt agggttaggg ttagggttag ggagacgaat tcggatccac 181 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt 241 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgca //