LOCUS       Exported                 887 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  EGFP under the control of CaMKIIa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKIIa-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    48..636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(101..121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(519..530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(867..886)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gcatggacga gctgtacaag taggaattcg atatcaagct tatcgataat caacctctgg
       61 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
      121 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
      181 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
      241 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
      301 ccaccacctg tcagctcctt tccggaactt tcgctttccc cctccctatt gccacggcgg
      361 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
      421 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
      481 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
      541 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
      601 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
      661 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
      721 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
      781 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
      841 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctgga
//
