LOCUS Exported 5863 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION retroviral expression of human c-Myc dN2 fragment. ACCESSION . VERSION . KEYWORDS pMXs-hu-c-Myc-dN2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5863) AUTHORS Nakagawa M, Takizawa N, Narita M, Ichisaka T, Yamanaka S TITLE Promotion of direct reprogramming by transformation-deficient Myc. JOURNAL Proc Natl Acad Sci U S A. 2010 Jul 26. ():. PUBMED 20660764 REFERENCE 2 (bases 1 to 5863) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5863 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1100..1516 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1446..1468 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1484..1500 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1526..1900 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1838..1854 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" protein_bind 1951..1975 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2833..2859 /codon_start=1 /product="nuclear localization signal of human c-Myc proto-oncogene (Dang and Lee, 1988)" /label=c-myc NLS /translation="PAAKRVKLD" CDS 3103..3132 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" protein_bind complement(3212..3236) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" LTR 3308..3901 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(3967..3984) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4138..4726) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4218..4237) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4890..5750) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5513..5532 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5751..5855) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca 1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg 1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac 1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg 1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc 1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc 1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac 1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct 1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca 1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca 1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt 1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct 1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt 1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt 1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggctccgcg 1981 gccgccccct tcaccatgcc cccggcgccc agcgaggata tctggaagaa attcgagctg 2041 ctgcccaccc cgcccctgtc ccctagccgc cgctccgggc tctgctcgcc ctcctacgtt 2101 gcggtcacac ccttctccct tcggggagac aacgacggcg gtggcgggag cttctccacg 2161 gccgaccagc tggagatggt gaccgagctg ctgggaggag acatggtgaa ccagagtttc 2221 atctgcgacc cggacgacga gaccttcatc aaaaacatca tcatccagga ctgtatgtgg 2281 agcggcttct cggccgccgc caagctcgtc tcagagaagc tggcctccta ccaggctgcg 2341 cgcaaagaca gcggcagccc gaaccccgcc cgcggccaca gcgtctgctc cacctccagc 2401 ttgtacctgc aggatctgag cgccgccgcc tcagagtgca tcgacccctc ggtggtcttc 2461 ccctaccctc tcaacgacag cagctcgccc aagtcctgcg cctcgcaaga ctccagcgcc 2521 ttctctccgt cctcggattc tctgctctcc tcgacggagt cctccccgca gggcagcccc 2581 gagcccctgg tgctccatga ggagacaccg cccaccacca gcagcgactc tgaggaggaa 2641 caagaagatg aggaagaaat cgatgttgtt tctgtggaaa agaggcaggc tcctggcaaa 2701 aggtcagagt ctggatcacc ttctgctgga ggccacagca aacctcctca cagcccactg 2761 gtcctcaaga ggtgccacgt ctccacacat cagcacaact acgcagcgcc tccctccact 2821 cggaaggact atcctgctgc caagagggtc aagttggaca gtgtcagagt cctgagacag 2881 atcagcaaca accgaaaatg caccagcccc aggtcctcgg acaccgagga gaatgtcaag 2941 aggcgaacac acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt 3001 gccctgcgtg accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc 3061 cttaaaaaag ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct 3121 gaagaggact tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg 3181 aactcttgtg cgtaaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtgatg 3241 agcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa 3301 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt 3361 ggaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag 3421 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct 3481 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca 3541 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc 3601 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg 3661 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc 3721 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag 3781 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc 3841 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat 3901 tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 3961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 4021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 4201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 4261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 4321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 4381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 4441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 4501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 4561 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 4681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4801 gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa 4861 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 4921 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 4981 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 5041 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 5101 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 5161 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 5221 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 5281 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 5341 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 5401 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 5461 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc 5521 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 5581 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 5641 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 5701 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 5761 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 5821 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttc //