LOCUS Exported 6020 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expresses photoconvertible dendra in macrophages. ACCESSION . VERSION . KEYWORDS Tol2-mpeg1-dendra2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6020) AUTHORS Harvie EA, Green JM, Neely MN, Huttenlocher A TITLE Innate immune response to Streptococcus iniae infection in zebrafish larvae. JOURNAL Infect Immun. 2013 Jan;81(1):110-21. doi: 10.1128/IAI.00642-12. Epub 2012 Oct 22. PUBMED 23090960 REFERENCE 2 (bases 1 to 6020) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6020 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(144..165) /label=F1ori-F /note="F1 origin, forward primer" CDS 2610..3302 /codon_start=1 /product="monomeric photoswitchable green-to-red fluorescent protein from Dendronephthya" /label=Dendra2 /note="mammalian codon-optimized" /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY SPLPSQVW" polyA_signal 3424..3545 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3461..3480) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3515..3534 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3836..3856) /label=T3 /note="T3 promoter, forward primer" promoter complement(3836..3854) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3875..3891) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3875..3891) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3888..3910) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3899..3915 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3923..3953) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3968..3989 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4106..4123) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4277..4865) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4357..4376) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5036..5896) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5659..5678 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5897..6001) /gene="bla" /label=AmpR promoter ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt 361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata 421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct 481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag 541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa 601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct 661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa 721 aaggctagca agatctgctc gagtgttgga gcacatctga catctgaaat tatttgaaag 781 cagcagaaat gtttttacag ctattacaaa atttacttaa caagaatcct ttaaaatatg 841 acaatttatt gccaataatt tacagtaagt tttgcagtac tttaatatta caaattatac 901 aactgtgctg gcacacaaaa aataacttaa tccagcacaa caacaacaac aacaactaca 961 aaaagcatat ttccttttgc tttttcttgt tcactcaact tttggaaaca tcaactgcac 1021 tgacctaaaa ttatacagta tgtcagtaat acaaaaacac ttagttaggt taacataaga 1081 ttattagttt tctggagagg tgaactactc tgtaagaaac ctttttaagt tgtgccaact 1141 aaacattttt atcagcagaa ctacaatgct tgatgagtga acaaaaaaac aaacaaacct 1201 tatttccttt tgttttttct tgtgtactta acattttttt cacctttatt tgaaacgaca 1261 gtagggagta tggacaggaa agtgttggga gaggagaggg atgagcatag aaccaggagg 1321 taggaatcaa actcaggtca ctatgaacac cggagtgcca ggtgtcaaca cactaaccac 1381 tacaccatca actccaacat ttacttgaaa acatcagttg agtgaacaaa aaactcttgt 1441 ggaaattggt actgagaaat aatacattat cacaacaaac aaaacagaga caaatgttaa 1501 caaaacaatt attagaaaca gaattagaaa ccggacagga ggtaaaacaa taatgataaa 1561 caagacaagg atcaaaacat ggcaggacaa agacagtttt ttgttcactc aacctcagat 1621 attttaggtc agtgcaactg atgtttacaa aggttgtatg catgtcaaca ccaatggtgt 1681 agtgattagt gtgttgacac aaggcactcc agtgttcaca ctgatgctgg tttgattccc 1741 acatcgatgt cctttgctaa tcctgcctct ctccccatgc tttcctgtct atactctcta 1801 ctgttgtgtc aattaaaggt gaaaaaagtt aagcacaaaa gaaaaatcta aggatataag 1861 gattatgttt ttttttgttc actcaaaatc aggcattcca gttctgcaga caaaaaatgt 1921 tcagttggca caacttaaaa agttttgtta cagagtagtt cacctctcca gaaaactaat 1981 aatcttatgt taacccaact aaatgttttt actgaaaaat aattgaatgt cagtgcagct 2041 gatgtttttt taaaagtgga gagaacaaaa aaagtgaaag gaaataagga ttcattgttg 2101 taccgactta tttttttgaa gaaaattctg ctatagggct ttcatgatcc cctatgctca 2161 cagcagtttt aggagatgct tataataaag aggatttgaa aaacctgcta atcttgtaca 2221 tttctaaaac aagaagccaa ccaatttcaa ttcctcctct ttcaaagcca atggaaaatt 2281 taaaacgcac agtctcttgc gtcatcaaaa ccattctgaa gactcatcaa aatccttcgt 2341 agctttaaat ataaatctat tattgaacag atcatacttc acaaaaaata taaatatatt 2401 tgattcattc acttcatgaa attgtccttc aacttaacgt ttgaacgcaa ggtaccaaaa 2461 cacaaagagg aacctcatag tctcatgacc cttcccccac ggatttgaaa ctgagaagtg 2521 gttttattga ggaactcagc aaaatctata atcaccatat ttgattgaaa atcacattag 2581 tgcaggagac agcaaaagga tccgccacca tgaacacccc gggaattaac ctgatcaagg 2641 aggacatgcg cgtgaaggtg cacatggagg gcaacgtgaa cggccacgcc ttcgtgatcg 2701 agggcgaggg caagggcaag ccctacgagg gcacccagac cgccaacctg accgtgaagg 2761 agggcgcccc cctgcccttc agctacgaca tcctgaccac cgccgtgcac tacggcaacc 2821 gggtgttcac caagtacccc gaggacatcc ccgactactt caagcagagc ttccccgagg 2881 gctacagctg ggagcgcacc atgaccttcg aggacaaggg catctgcacc atccgcagcg 2941 acatcagcct ggagggcgac tgcttcttcc agaacgtgcg cttcaagggc accaacttcc 3001 cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaagc 3061 tgcacgtgcg cgacggcctg ctggtgggca acatcaacat ggccctgctg ctggagggcg 3121 gcggccacta cctgtgcgac ttcaagacca cctacaaggc caagaaggtg gtgcagctgc 3181 ccgacgccca cttcgtggac caccgcatcg agatcctggg caacgacagc gactacaaca 3241 aggtgaagct gtacgagcac gccgtggccc gctacagccc cctgcccagc caggtgtggt 3301 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 3361 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 3421 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3541 tcttaaggcg agatcctgcg gccgcaaggg aaaatagaat gaagtgatct ccaaaaaata 3601 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa 3661 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc 3721 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct 3781 ggttcttgac cccctacctt cagcaagccc agcagatcca ctagatcttt tgttcccttt 3841 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4021 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4081 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 5341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 5401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 5461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 5521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 5581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 5641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 5701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 5761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 5821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6001 gcacatttcc ccgaaaagtg //