LOCUS       Exported                 800 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  expression plasmid for C-terminal his tagged S. aureus SrtA with 
            enhanced catalytic activity.
ACCESSION   .
VERSION     .
KEYWORDS    pet30b-5M SrtA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Shi J, Kundrat L, Pishesha N, Bilate A, Theile C, Maruyama T, Dougan
            SK, Ploegh HL, Lodish HF
  TITLE     Engineered red blood cells as carriers for systemic delivery of a 
            wide array of functional probes.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10131-6. doi: 
            10.1073/pnas.1409861111. Epub 2014 Jun 30.
  PUBMED    24982154
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             482..499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(552..570)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      566..613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaagct aaacctcaaa ttccgaaaga
       61 taaatcaaaa gtggcaggct atattgaaat tccagatgct gatattaaag aaccagtata
      121 tccaggacca gcaacacgcg aacaattaaa tagaggtgta agctttgcag aagaaaatga
      181 atcactagat gatcaaaata tttcaattgc aggacacact ttcattgacc gtccgaacta
      241 tcaatttaca aatcttaaag cagccaaaaa aggtagtatg gtgtacttta aagttggtaa
      301 tgaaacacgt aagtataaaa tgacaagtat aagaaacgtt aagccaacag ctgtagaagt
      361 tctagatgaa caaaaaggta aagataaaca attaacatta attacttgtg atgattacaa
      421 tgaagagaca ggcgtttggg aaacacgtaa aatctttgta gctacagaag tcaaactcga
      481 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      541 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      601 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa
//
