LOCUS Exported 13237 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutively express bud neck localized PhyB in Saccharomyces cerevisiae. ACCESSION . VERSION . KEYWORDS PhyB-mCherry-CDC10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13237) AUTHORS Yang X, Jost AP, Weiner OD, Tang C TITLE A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. JOURNAL Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub 2013 Jun 12. PUBMED 23761071 REFERENCE 2 (bases 1 to 13237) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13237 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 276..683 /gene="S. cerevisiae LEU2" /label=LEU2 promoter promoter 3292..3996 /gene="S. cerevisiae ADH1" /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 6786..7490 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE GRHSTGGMDELYK" primer_bind complement(6928..6946) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 7196..7215 /label=mCherry-F /note="mCherry, forward primer" primer_bind complement(9784..9803) /label=T7 /note="T7 promoter, forward primer" promoter complement(9785..9803) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9810..9827) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9810..9826) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9819..9841) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9967..10422 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10054..10073) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10264..10285 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(10778..10797) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10897..10919 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(10957..10975) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 11060..11164 /gene="bla" /label=AmpR promoter CDS 11165..12025 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(11383..11402) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 12196..12784 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 12685..12704 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12938..12955 /label=L4440 /note="L4440 vector, forward primer" protein_bind 13072..13093 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 13108..13138 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13146..13162 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 13151..13173 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 13170..13186 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 13170..13186 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 13207..13225 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta 61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca 121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat 181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta 241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt 301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac 361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca 421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga 481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat 541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt 601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata 661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa 721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa 781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc 841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg 901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa 961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt 1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct 1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc 1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat 1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga 1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa 1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga 1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc 1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt 1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc 1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg 1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga 1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca 1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag 1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca 1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg 1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc 1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc 2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa 2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga 2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc 2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag 2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga 2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc 2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat 2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag 2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat 2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg 2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc 2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg 2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc 2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact 2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga 2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca 3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag 3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag 3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta 3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca 3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg 3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg 3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa 3421 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct 3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat 3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc 3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa 3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca 3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc 3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt 3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta 3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc 3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaaa gcaatggttt 4021 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt 4081 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga 4141 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg 4201 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact 4261 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag 4321 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg 4381 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga 4441 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg 4501 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag 4561 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg 4621 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag 4681 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt 4741 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa 4801 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc 4861 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt 4921 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa 4981 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt 5041 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg 5101 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg 5161 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc 5221 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc 5281 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac 5341 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa 5401 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt 5461 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg 5521 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg 5581 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat 5641 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat 5701 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt 5761 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg 5821 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg 5881 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg 5941 aacaggggat tgatgagtta ggtgcagttg caagagagat ggttaggctc attgagactg 6001 caactgttcc tatattcgct gtggatgccg gaggctgcat caatggatgg aacgctaaga 6061 ttgcagagtt gacaggtctc tcagttgaag aagctatggg gaagtctctg gtttctgatt 6121 taatatacaa agagaatgaa gcaactgtca ataagcttct ttctcgtgct ttgagagggg 6181 acgaggaaaa gaatgtggag gttaagctga aaactttcag ccccgaacta caagggaaag 6241 cagtttttgt ggttgtgaat gcttgttcca gcaaggacta cttgaacaac attgtcggcg 6301 tttgttttgt tggacaagac gttactagtc agaaaatcgt aatggataag ttcatcaaca 6361 tacaaggaga ttacaaggct attgtacata gcccaaaccc tctaatcccg ccaatttttg 6421 ctgctgacga gaacacgtgc tgcctggaat ggaacatggc gatggaaaag cttacgggtt 6481 ggtctcgcag tgaagtgatt gggaaaatga ttgtcgggga agtgtttggg agctgttgca 6541 tgctaaaggg tcctgatgct ttaaccaagt tcatgattgt attgcataat gcgattggtg 6601 gccaagatac ggataagttc cctttcccat tctttgaccg caatgggaag tttgttcagg 6661 ctctattgac tgcaaacaag cgggttagcc tcgagggaaa ggttattggg gctttctgtt 6721 tcttgcaaat cccgagcgaa ttcgatagtg ctggtagtgc tggtagtgct ggtggtagtt 6781 ccctagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca 6841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 6901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc 6961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc 7021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc 7081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg 7141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg 7201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg 7261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg 7321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg 7381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 7441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcgata 7501 gcgcaggcag tgctggtaag gctagcatgg atcctctcag ctcagtacag cctgcttctt 7561 atgttggttt tgataccatc acgaatcaga tcgaacatcg tctgttgaag aaaggttttc 7621 aatttaatat aatggttgtt ggccaatccg gattgggtaa aagtactcta ataaatacgt 7681 tatttgcctc acatttgatt gattctgcta ctggtgatga tatttctgcc ctgcctgtta 7741 caaaaacaac tgaaatgaaa atttctactc atactcttgt ggaggaccgc gttcgcttga 7801 atattaatgt tatagataca cctggatttg gtgactttat tgacaattct aaagcttggg 7861 agcctattgt gaagtacatt aaggaacaac attctcaata cttacgtaaa gaattgacag 7921 cccaacgtga aaggtttatt actgatacaa gagttcatgc aattctttat ttcctgcaac 7981 caaatggaaa ggagttgagc cgccttgacg ttgaagcctt gaaaagattg acagaaatag 8041 caaatgttat accagttatt ggcaagtcgg atacattgac tttagatgaa agaacggagt 8101 ttagggagct tattcaaaat gaattcgaaa aatacaattt caagatttat ccttatgatt 8161 cggaagaact aactgacgag gaattagaac taaacagaag tgttagatct atcattccgt 8221 ttgcagtggt tggttctgag aatgagattg aaataaacgg tgaaaccttc aggggaagaa 8281 aaactcgttg gagcgctatt aatgttgagg atatcaacca gtgtgatttt gtatatttaa 8341 gggaattttt gattcgaact catctccaag acttaatcga aacaacttcc tacattcatt 8401 atgaagggtt cagagcaaga caattaattg ccttgaaaga aaatgcgaat agtcgttcct 8461 cagctcatat gtctagcaac gccattcaac gttgagcggc cgccaccgcg gtggagctct 8521 aagcaaatag ctaaattata tacgaattaa tattatgatt aagtgtttac gtgagtgcga 8581 tatttttatt actatcttat acagttgtat atactctata aaatgagttg tctattaatt 8641 aacgcgatga atgctttctg ggtttacctc tccaacaact ctagtttact tctcaataca 8701 ttcaattgta tttgatttgt caatacttca tcattaatca attctatagt tttgtttttc 8761 tcgtttattt ccaaatttaa tgcatcaatt ttattattca atttgtcgtt gattttggtt 8821 aatgatttta tggtttgatc tctggcattg attgtttgtg ttagtttttc attattgata 8881 attaaattat ttaagttagt tatcaactcg gtgttttcaa gtttcaagtt ttcaatttct 8941 ttagagttta ttagatttgt caaagtttct gaattgcttg attggtcctg tagaagagta 9001 tttgttgttg tggataattg attcaatttt tgagacaatt gctggaaggc gttgaaatat 9061 ctagcatcaa tctcatggtt tttttcccga gagtctcgta gattcaattg ttttaatata 9121 tcttgggacc actcttgatt tgaactcatg gaaattaaac tgggtgttgt gttgtggtgt 9181 aatgattgta ccccctttgc ttataattgt gtggccaccg aagtcggtga tgctgtcgcc 9241 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata 9301 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca 9361 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag 9421 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag 9481 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta 9541 acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa 9601 aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa 9661 taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag 9721 aaacggcctt aacgacgtag gccggccatt taaatgttta aacggccacg ctggcccaat 9781 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg 9841 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 9901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 9961 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 10021 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 10081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 10141 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 10201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 10261 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 10321 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 10381 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct 10441 ccttacgcat ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg 10501 aagctctaat ttgtgagttt agtattagga tctctaccct tggcgaaaag tcctctgcca 10561 acaatgatga tatctgatcc accacttaca acttcgtcga cggttctgta ctgctgaccc 10621 aatatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg 10681 gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa 10741 aatgatgaat tgaaaaggtg gtatggtgca ctctcagtac aatctgctct gatgccgcat 10801 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 10861 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 10921 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 10981 aggttaatgt catgataata atggtttctt agacgtcggc gcgccgagga cgtcaggtgg 11041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 11101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 11161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 11221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 11281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 11341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 11401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 11461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 11521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 11581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 11641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 11701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 11761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 11821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 11881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 11941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 12001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 12061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 12121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 12181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 12241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 12301 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 12361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 12421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 12481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 12541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 12601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 12661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 12721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 12781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 12841 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 12901 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 12961 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 13021 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 13081 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg 13141 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 13201 gctcggaatt aaccctcact aaagggaaca aaagctg //