LOCUS Exported 721 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION gateway adapted mammalian expression vector. ACCESSION . VERSION . KEYWORDS pBABEpuro-gateway SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 721) AUTHORS Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, Meyerson M TITLE Functional analysis of receptor tyrosine kinase mutations in lung cancer identifies oncogenic extracellular domain mutations of ERBB2. JOURNAL Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug 20. PUBMED 22908275 REFERENCE 2 (bases 1 to 721) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..721 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(109..233) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(270..290) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 275..604 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 455..590 /label=SV40 ori /note="SV40 origin of replication" primer_bind 517..536 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(614..633) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" ORIGIN 1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg 121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata 181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatgtag 241 gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc 301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tangcttttg 601 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 661 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 721 c //