LOCUS       Exported                1073 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Doxycycline-inducible lentiviral expression of SpCas9.
ACCESSION   .
VERSION     .
KEYWORDS    pCW-Cas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Wang T, Wei JJ, Sabatini DM, Lander ES
  TITLE     Genetic screens in human cells using the CRISPR-Cas9 system.
  JOURNAL   Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. 
            Epub 2013 Dec 12.
  PUBMED    24336569
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      8..17
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             17..82
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             89..109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggctagcgcc accatggact ataaggacca cgacggagac tacaaggatc atgatattga
       61 ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg
      121 agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg
      181 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
      241 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
      301 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
      361 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
      421 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
      481 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
      541 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
      601 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
      661 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
      721 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
      781 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
      841 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
      901 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
      961 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     1021 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca agg
//
