LOCUS Exported 1116 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Doxycycline-inducible lentiviral expression of SpCas9. ACCESSION . VERSION . KEYWORDS pCW-Cas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1116) AUTHORS Wang T, Wei JJ, Sabatini DM, Lander ES TITLE Genetic screens in human cells using the CRISPR-Cas9 system. JOURNAL Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. PUBMED 24336569 REFERENCE 2 (bases 1 to 1116) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1116 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 17..37 /label=T3 /note="T3 promoter, forward primer" promoter 19..37 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 65..291 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 292..472 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 519..644 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" ORIGIN 1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc 61 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 121 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 181 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 241 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 301 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 361 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 421 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 481 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 541 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 601 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 661 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 721 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 781 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 841 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 901 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 961 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 1021 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1081 gcacccacca aggcaaagag aagagtggtg cagaga //