LOCUS Exported 10119 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Yeast marker swap plasmid for converting LEU2 to LYS2. ACCESSION . VERSION . KEYWORDS D771 leu2::LYS2 Disruptor Converter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10119) AUTHORS Voth WP, Jiang YW, Stillman DJ TITLE New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. JOURNAL Yeast. 2003 Aug;20(11):985-93. PUBMED 12898713 REFERENCE 2 (bases 1 to 10119) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10119 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 364..386 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 378..395 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 1746..1985 /gene="S. cerevisiae LYS2" /label=LYS2 promoter CDS 1986..6164 /codon_start=1 /gene="S. cerevisiae LYS2" /product="alpha-aminoadipate reductase, required for lysine biosynthesis" /label=LYS2 /note="yeast auxotrophic marker" /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA" primer_bind complement(7898..7914) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7898..7914) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7911..7933) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7922..7938 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7946..7976) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7991..8012 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8129..8146) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8300..8888) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8380..8399) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9059..9919) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9682..9701 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9920..10024) /gene="bla" /label=AmpR promoter primer_bind 10092..10110 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcgcga gctcgaggag 421 aacttctagt atatccacat acctaatatt attgccttat taaaaatgga atcccaacaa 481 ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt 541 gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag taattggttg 601 tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg 661 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttt 721 ctctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg 781 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg 841 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt 901 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg 961 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt 1021 tcagcaatat atatatatat atatatatat atttcaagga tataccattg taatgtctgc 1081 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc 1141 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa 1201 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct 1261 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg 1321 taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta 1381 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa 1441 gccacaattt gctaaaggta ctgacttcgt tgttgttaga gaattagtgg gaggtattta 1501 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac 1561 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc 1621 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag 1681 aaaaactgtg gaggaaacca tcaagaacga attctcgacg gatccggtga ttgattgagc 1741 aagctaattc cacttgcaat tacataaaaa attccggcgg tttttcgcgt gtgactcaat 1801 gtcgaaatac ctgcctaatg aacatgaaca tcgcccaaat gtatttgaag acccgctggg 1861 agaagttcaa gatatataag taacaagcag ccaatagtat aaaaaaaaat ctgagtttat 1921 tacctttcct ggaatttcag tgaaaaactg ctaattatag agagatatca cagagttact 1981 cactaatgac taacgaaaag gtctggatag agaagttgga taatccaact ctttcagtgt 2041 taccacatga ctttttacgc ccacaacaag aaccttatac gaaacaagct acatattcgt 2101 tacagctacc tcagctcgat gtgcctcatg atagtttttc taacaaatac gctgtcgctt 2161 tgagtgtatg ggctgcattg atatatagag taaccggtga cgatgatatt gttctttata 2221 ttgcgaataa caaaatctta agattcaata ttcaaccaac gtggtcattt aatgagctgt 2281 attctacaat taacaatgag ttgaacaagc tcaattctat tgaggccaat ttttcctttg 2341 acgagctagc tgaaaaaatt caaagttgcc aagatctgga aaggacccct cagttgttcc 2401 gtttggcctt tttggaaaac caagatttca aattagacga gttcaagcat catttagtgg 2461 actttgcttt gaatttggat accagtaata atgcgcatgt tttgaactta atttataaca 2521 gcttactgta ttcgaatgaa agagtaacca ttgttgcgga ccaatttact caatatttga 2581 ctgctgcgct aagcgatcca tccaattgca taactaaaat ctctctgatc accgcatcat 2641 ccaaggatag tttacctgat ccaactaaga acttgggctg gtgcgatttc gtggggtgta 2701 ttcacgacat tttccaggac aatgctgaag ccttcccaga gagaacctgt gttgtggaga 2761 ctccaacact aaattccgac aagtcccgtt ctttcactta tcgcgacatc aaccgcactt 2821 ctaacatagt tgcccattat ttgattaaaa caggtatcaa aagaggtgat gtagtgatga 2881 tctattcttc taggggtgtg gatttgatgg tatgtgtgat gggtgtcttg aaagccggcg 2941 caaccttttc agttatcgac cctgcatatc ccccagccag acaaaccatt tacttaggtg 3001 ttgctaaacc acgtgggttg attgttatta gagctgctgg acaattggat caactagtag 3061 aagattacat caatgatgaa ttggagattg tttcaagaat caattccatc gctattcaag 3121 aaaatggtac cattgaaggt ggcaaattgg acaatggcga ggatgttttg gctccatatg 3181 atcactacaa agacaccaga acaggtgttg tagttggacc agattccaac ccaaccctat 3241 ctttcacatc tggttccgaa ggtattccta agggtgttct tggtagacat ttttccttgg 3301 cttattattt caattggatg tccaaaaggt tcaacttaac agaaaatgat aaattcacaa 3361 tgctgagcgg tattgcacat gatccaattc aaagagatat gtttacacca ttatttttag 3421 gtgcccaatt gtatgtccct actcaagatg atattggtac accgggccgt ttagcggaat 3481 ggatgagtaa gtatggttgc acagttaccc atttaacacc tgccatgggt caattactta 3541 ctgcccaagc tactacacca ttccctaagt tacatcatgc gttctttgtg ggtgacattt 3601 taacaaaacg tgattgtctg aggttacaaa ccttggcaga aaattgccgt attgttaata 3661 tgtacggtac cactgaaaca cagcgtgcag tttcttattt cgaagttaaa tcaaaaaatg 3721 acgatccaaa ctttttgaaa aaattgaaag atgtcatgcc tgctggtaaa ggtatgttga 3781 acgttcagct actagttgtt aacaggaacg atcgtactca aatatgtggt attggcgaaa 3841 taggtgagat ttatgttcgt gcaggtggtt tggccgaagg ttatagagga ttaccagaat 3901 tgaataaaga aaaatttgtg aacaactggt ttgttgaaaa agatcactgg aattatttgg 3961 ataaggataa tggtgaacct tggagacaat tctggttagg tccaagagat agattgtaca 4021 gaacgggtga tttaggtcgt tatctaccaa acggtgactg tgaatgttgc ggtagggctg 4081 atgatcaagt taaaattcgt gggttcagaa tcgaattagg agaaatagat acgcacattt 4141 cccaacatcc attggtaaga gaaaacatta ctttagttcg caaaaatgcc gacaatgagc 4201 caacattgat cacatttatg gtcccaagat ttgacaagcc agatgacttg tctaagttcc 4261 aaagtgatgt tccaaaggag gttgaaactg accctatagt taagggctta atcggttacc 4321 atcttttatc caaggacatc aggactttct taaagaaaag attggctagc tatgctatgc 4381 cttccttgat tgtggttatg gataaactac cattgaatcc aaatggtaaa gttgataagc 4441 ctaaacttca attcccaact cccaagcaat taaatttggt agctgaaaat acagtttctg 4501 aaactgacga ctctcagttt accaatgttg agcgcgaggt tagagactta tggttaagta 4561 tattacctac caagccagca tctgtatcac cagatgattc gtttttcgat ttaggtggtc 4621 attctatctt ggctaccaaa atgattttta ccttaaagaa aaagctgcaa gttgatttac 4681 cattgggcac aattttcaag tatccaacga taaaggcctt tgccgcggaa attgacagaa 4741 ttaaatcatc gggtggatca tctcaaggtg aggtcgtcga aaatgtcact gcaaattatg 4801 cggaagacgc caagaaattg gttgagacgc taccaagttc gtacccctct cgagaatatt 4861 ttgttgaacc taatagtgcc gaaggaaaaa caacaattaa tgtgtttgtt accggtgtca 4921 caggatttct gggctcctac atccttgcag atttgttagg acgttctcca aagaactaca 4981 gtttcaaagt gtttgcccac gtcagggcca aggatgaaga agctgcattt gcaagattac 5041 aaaaggcagg tatcacctat ggtacttgga acgaaaaatt tgcctcaaat attaaagttg 5101 tattaggcga tttatctaaa agccaatttg gtctttcaga tgagaagtgg atggatttgg 5161 caaacacagt tgatataatt atccataatg gtgcgttagt tcactgggtt tatccatatg 5221 ccaaattgag ggatccaaat gttatttcaa ctatcaatgt tatgagctta gccgccgtcg 5281 gcaagccaaa gttctttgac tttgtttcct ccacttctac tcttgacact gaatactact 5341 ttaatttgtc agataaactt gttagcgaag ggaagccagg cattttagaa tcagacgatt 5401 taatgaactc tgcaagcggg ctcactggtg gatatggtca gtccaaatgg gctgctgagt 5461 acatcattag acgtgcaggt gaaaggggcc tacgtgggtg tattgtcaga ccaggttacg 5521 taacaggtgc ctctgccaat ggttcttcaa acacagatga tttcttattg agatttttga 5581 aaggttcagt ccaattaggt aagattccag atatcgaaaa ttccgtgaat atggttccag 5641 tagatcatgt tgctcgtgtt gttgttgcta cgtctttgaa tcctcccaaa gaaaatgaat 5701 tggccgttgc tcaagtaacg ggtcacccaa gaatattatt caaagactac ttgtatactt 5761 tacacgatta tggttacgat gtcgaaatcg aaagctattc taaatggaag aaatcattgg 5821 aggcgtctgt tattgacagg aatgaagaaa atgcgttgta tcctttgcta cacatggtct 5881 tagacaactt acctgaaagt accaaagctc cggaactaga cgataggaac gccgtggcat 5941 ctttaaagaa agacaccgca tggacaggtg ttgattggtc taatggaata ggtgttactc 6001 cagaagaggt tggtatatat attgcatttt taaacaaggt tggattttta cctccaccaa 6061 ctcataatga caaacttcca ctgccaagta tagaactaac tcaagcgcaa ataagtctag 6121 ttgcttcagg tgctggtgct cgtggaagct ccgcagcagc ttaaggttga gcattacgta 6181 tgatatgtcc atgtacaata attaaatatg aattaggaga aagacttagc ttcttttcgg 6241 gtgatgtcac ttaaaaactc cgagaataat atataataag agaataaaat attagttatt 6301 gaataagaac tgtaaatcag ctggcgttag tctgctaatg gcagcttcat cttggtttat 6361 tgtagcatga atcatatttg cctttttttc ctgtaattca atgattcttg cttctatact 6421 atcctcaatg caaaaccttg tgatcttcac aggtcgatac tgaccaattc tatgaactct 6481 atcaccactt tgccattcaa cactagggtt ccaccatggg tctaaaatga atacttgcga 6541 agcttcacaa agattcaaag caacaccgcc cgcctttaaa ctgaccaaga aaacctcgca 6601 ttgaatgttg ttcatgaaat acttgatggt ttcatctctt tgcgtcggtg acatactacc 6661 ctgaagcttc actgtttgaa atccagctct tttcaatctc cactctacca gatccagcat 6721 actggtaaac tgggaaaaca caatggattt aatcgttctc ttgttgcttc tcagtttgta 6781 tagttcttcc acaagtgctt cgattttcgt tgatgattgc cacttgccac tcatgtttag 6841 acggctaaca atactttgct ttttgaagga atcaaggtcc acttccaaag caggttgaga 6901 caaatcgagc ttgctcaatc aatcaccgga tccccgggaa ttccctacat tgaaggttca 6961 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg 7021 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc 7081 aggctccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc 7141 atttggtttg tacgaaccat gccatggttc cgctccagat ttgccaaaga ataaggtcaa 7201 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga 7261 agaaggtaaa gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg 7321 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa 7381 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa 7441 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg 7501 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga 7561 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt 7621 aacattaata ttgacaagga ggagggcatc acacaaaaag ttaggtgtaa cagaaaatca 7681 tgaaactatg atttctaatt tatatattgg aggattttct ctaaaaaaaa aaatacaaca 7741 aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac cttcttgaac 7801 catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa cggccttacg 7861 acgtagtcga cctgcagcca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 7921 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7981 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 8041 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 8101 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8161 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8221 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8281 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8341 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8401 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8461 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt 8521 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8581 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8641 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8701 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 8761 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8821 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8881 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8941 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 9001 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 9061 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9121 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9181 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9241 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9301 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9361 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9421 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9481 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9541 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9601 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9661 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9721 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9781 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9841 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9901 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9961 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 10021 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 10081 taacctataa aaataggcgt atcacgaggc cctttcgtc //