LOCUS Exported 939 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.. ACCESSION . VERSION . KEYWORDS pACT2-CAS9c SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 939) AUTHORS Gao Y, Zhao Y TITLE Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. JOURNAL J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. PUBMED 24373158 REFERENCE 2 (bases 1 to 939) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..939 /organism="synthetic DNA construct" /mol_type="other DNA" ORIGIN 1 tgctatcaag tataaataga cctgcaatta ttaatctttt gtttcctcgt cattgttctc 61 gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc aagcatacaa 121 tcaactccaa tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc 181 tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat 241 accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag 301 acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat 361 cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc 421 ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca 481 atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat 541 ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg 601 ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac 661 aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa 721 gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc 781 aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg 841 tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac 901 ctggccgaag atgccaagct tcaactgagc aaagacacc //