LOCUS       Exported                9556 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  multicopy (YEp) vector with a LYS2 marker and a 2-um origin of 
            replication.
ACCESSION   .
VERSION     .
KEYWORDS    pRS327
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Eriksson P, Thomas LR, Thorburn A, Stillman DJ
  TITLE     pRS yeast vectors with a LYS2 marker.
  JOURNAL   Biotechniques. 2004 Feb;36(2):212-3.
  PUBMED    14989082
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(717..4895)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(4896..5135)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     rep_origin      complement(5189..5644)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5326..5347)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5538..5557
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5770..5792
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5784..5801
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5785..5801
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5808..5827
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5808..5826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    5835..5942
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     5859..5875
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5859..5875
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5909..5925)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5910..5926)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(5954..5972)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5993..6009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5993..6009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6006..6028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6017..6033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6041..6071)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6086..6107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6224..6241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6395..6983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7154..8014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7777..7796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8015..8119)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8146..9488
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9109..9156)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9529..9547
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgca ccatatagct tcgcaagtat tcattttaga
      361 cccatggtgg aaccctagtg ttgaatggca aagtggtgat agagttcata gaattggtca
      421 gtatcgacct gtgaagatca caaggttttg cattgaggat agtatagaag caagaatcat
      481 tgaattacag gaaaaaaagg caaatatgat tcatgctaca ataaaccaag atgaagctgc
      541 cattagcaga ctaacgccag ctgatttaca gttcttattc aataactaat attttattct
      601 cttattatat attattctcg gagtttttaa gtgacatcac ccgaaaagaa gctaagtctt
      661 tctcctaatt catatttaat tattgtacat ggacatatca tacgtaatgc tcaaccttaa
      721 gctgctgcgg agcttccacg agcaccagca cctgaagcaa ctagacttat ttgcgcttga
      781 gttagttcta tacttggcag tggaagtttg tcattatgag ttggtggagg taaaaatcca
      841 accttgttta aaaatgcaat atatatacca acctcttctg gagtaacacc tattccatta
      901 gaccaatcaa cacctgtcca tgcggtgtct ttctttaaag atgccacggc gttcctatcg
      961 tctagttccg gagctttggt actttcaggt aagttgtcta agaccatgtg tagcaaagga
     1021 tacaacgcat tttcttcatt cctgtcaata acagacgcct ccaatgattt cttccattta
     1081 gaatagcttt cgatttcgac atcgtaacca taatcgtgta aagtatacaa gtagtctttg
     1141 aataatattc ttgggtgacc cgttacttga gcaacggcca attcattttc tttgggagga
     1201 ttcaaagacg tagcaacaac aacacgagca acatgatcta ctggaaccat attcacggaa
     1261 ttttcgatat ctggaatctt acctaattgg actgaacctt tcaaaaatct caataagaaa
     1321 tcatctgtgt ttgaagaacc attggcagag gcacctgtta cgtaacctgg tctgacaata
     1381 cacccacgta ggcccctttc acctgcacgt ctaatgatgt actcagcagc ccatttggac
     1441 tgaccatatc caccagtgag cccgcttgca gagttcatta aatcgtctga ttctaaaatg
     1501 cctggcttcc cttcgctaac aagtttatct gacaaattaa agtagtattc agtgtcaaga
     1561 gtagaagtgg aggaaacaaa gtcaaagaac tttggcttgc cgacggcggc taagctcata
     1621 acattgatag ttgaaataac atttggatcc ctcaatttgg catatggata aacccagtga
     1681 actaacgcac cattatggat aattatatca actgtgtttg ccaaatccat ccacttctca
     1741 tctgaaagac caaattggct tttagataaa tcgcctaata caactttaat atttgaggca
     1801 aatttttcgt tccaagtacc ataggtgata cctgcctttt gtaatcttgc aaatgcagct
     1861 tcttcatcct tggccctgac gtgggcaaac actttgaaac tgtagttctt tggagaacgt
     1921 cctaacaaat ctgcaaggat gtaggagccc agaaatcctg tgacaccggt aacaaacaca
     1981 ttaattgttg tttttccttc ggcactatta ggttcaacaa aatattctcg agaggggtac
     2041 gaacttggta gcgtctcaac caatttcttg gcgtcttccg cataatttgc agtgacattt
     2101 tcgacgacct caccttgaga tgatccaccc gatgatttaa ttctgtcaat ttccgcggca
     2161 aaggccttta tcgttggata cttgaaaatt gtgcccaatg gtaaatcaac ttgcagcttt
     2221 ttctttaagg taaaaatcat tttggtagcc aagatagaat gaccacctaa atcgaaaaac
     2281 gaatcatctg gtgatacaga tgctggcttg gtaggtaata tacttaacca taagtctcta
     2341 acctcgcgct caacattggt aaactgagag tcgtcagttt cagaaactgt attttcagct
     2401 accaaattta attgcttggg agttgggaat tgaagtttag gcttatcaac tttaccattt
     2461 ggattcaatg gtagtttatc cataaccaca atcaaggaag gcatagcata gctagccaat
     2521 cttttcttta agaaagtcct gatgtccttg gataaaagat ggtaaccgat taagccctta
     2581 actatagggt cagtttcaac ctcctttgga acatcacttt ggaacttaga caagtcatct
     2641 ggcttgtcaa atcttgggac cataaatgtg atcaatgttg gctcattgtc ggcatttttg
     2701 cgaactaaag taatgttttc tcttaccaat ggatgttggg aaatgtgcgt atctatttct
     2761 cctaattcga ttctgaaccc acgaatttta acttgatcat cagccctacc gcaacattca
     2821 cagtcaccgt ttggtagata acgacctaaa tcacccgttc tgtacaatct atctcttgga
     2881 cctaaccaga attgtctcca aggttcacca ttatccttat ccaaataatt ccagtgatct
     2941 ttttcaacaa accagttgtt cacaaatttt tctttattca attctggtaa tcctctataa
     3001 ccttcggcca aaccacctgc acgaacataa atctcaccta tttcgccaat accacatatt
     3061 tgagtacgat cgttcctgtt aacaactagt agctgaacgt tcaacatacc tttaccagca
     3121 ggcatgacat ctttcaattt tttcaaaaag tttggatcgt cattttttga tttaacttcg
     3181 aaataagaaa ctgcacgctg tgtttcagtg gtaccgtaca tattaacaat acggcaattt
     3241 tctgccaagg tttgtaacct cagacaatca cgttttgtta aaatgtcacc cacaaagaac
     3301 gcatgatgta acttagggaa tggtgtagta gcttgggcag taagtaattg acccatggca
     3361 ggtgttaaat gggtaactgt gcaaccatac ttactcatcc attccgctaa acggcccggt
     3421 gtaccaatat catcttgagt agggacatac aattgggcac ctaaaaataa tggtgtaaac
     3481 atatctcttt gaattggatc atgtgcaata ccgctcagca ttgtgaattt atcattttct
     3541 gttaagttga accttttgga catccaattg aaataataag ccaaggaaaa atgtctacca
     3601 agaacaccct taggaatacc ttcggaacca gatgtgaaag atagggttgg gttggaatct
     3661 ggtccaacta caacacctgt tctggtgtct ttgtagtgat catatggagc caaaacatcc
     3721 tcgccattgt ccaatttgcc accttcaatg gtaccatttt cttgaatagc gatggaattg
     3781 attcttgaaa caatctccaa ttcatcattg atgtaatctt ctactagttg atccaattgt
     3841 ccagcagctc taataacaat caacccacgt ggtttagcaa cacctaagta aatggtttgt
     3901 ctggctgggg gatatgcagg gtcgataact gaaaaggttg cgccggcttt caagacaccc
     3961 atcacacata ccatcaaatc cacaccccta gaagaataga tcatcactac atcacctctt
     4021 ttgatacctg ttttaatcaa ataatgggca actatgttag aagtgcggtt gatgtcgcga
     4081 taagtgaaag aacgggactt gtcggaattt agtgttggag tctccacaac acaggttctc
     4141 tctgggaagg cttcagcatt gtcctggaaa atgtcgtgaa tacaccccac gaaatcgcac
     4201 cagcccaagt tcttagttgg atcaggtaaa ctatccttgg atgatgcggt gatcagagag
     4261 attttagtta tgcaattgga tggatcgctt agcgcagcag tcaaatattg agtaaattgg
     4321 tccgcaacaa tggttactct ttcattcgaa tacagtaagc tgttataaat taagttcaaa
     4381 acatgcgcat tattactggt atccaaattc aaagcaaagt ccactaaatg atgcttgaac
     4441 tcgtctaatt tgaaatcttg gttttccaaa aaggccaaac ggaacaactg aggggtcctt
     4501 tccagatctt ggcaactttg aattttttca gctagctcgt caaaggaaaa attggcctca
     4561 atagaattga gcttgttcaa ctcattgtta attgtagaat acagctcatt aaatgaccac
     4621 gttggttgaa tattgaatct taagattttg ttattcgcaa tataaagaac aatatcatcg
     4681 tcaccggtta ctctatatat caatgcagcc catacactca aagcgacagc gtatttgtta
     4741 gaaaaactat catgaggcac atcgagctga ggtagctgta acgaatatgt agcttgtttc
     4801 gtataaggtt cttgttgtgg gcgtaaaaag tcatgtggta acactgaaag agttggatta
     4861 tccaacttct ctatccagac cttttcgtta gtcattagtg agtaactctg tgatatctct
     4921 ctataattag cagtttttca ctgaaattcc aggaaaggta ataaactcag attttttttt
     4981 atactattgg ctgcttgtta cttatatatc ttgaacttct cccagcgggt cttcaaatac
     5041 atttgggcga tgttcatgtt cattaggcag gtatttcgac attgagtcac acgcgaaaaa
     5101 ccgccggaat tttttatgta attgcaagtg gaattatatg cggtgtgaaa taccgcacag
     5161 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc
     5221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     5401 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     5461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     5521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5641 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     5701 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     5761 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
     5821 tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg
     5881 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
     5941 ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc
     6001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6061 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6241 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctcggc ccccctgacg
     6421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6481 accaggcgtt cccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     6601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7201 ttcgttcatc catagttgcc tgactgcccg tcgtgtagat aactacgata cgggagggct
     7261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7561 tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg
     8161 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     8221 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     8281 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     8341 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     8401 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     8461 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     8521 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     8581 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     8641 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     8701 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     8761 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     8821 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     8881 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     8941 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     9001 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
     9061 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     9121 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
     9181 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
     9241 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
     9301 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
     9361 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
     9421 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
     9481 attggatcat actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9541 acgaggccct ttcgtc
//
