LOCUS       Exported                 842 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  FX cloning insect cell expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    pFBXC3H
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 842)
  TITLE     Weinert et al, Grutter lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(161..190)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(191..214)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             241..261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             265..285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    304..428
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        453..483
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(603..622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagtact
      121 tctcgacaag cttgtcgaga ctgcaggctc tagattatta atgatgatga tgatggtgat
      181 gatgatggtg tggaccttga aacaaaactt ctaatgcaga agagctgaac tagtggatcc
      241 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagctag cgaattcgat
      301 atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      361 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      421 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
      481 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
      541 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
      601 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
      661 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
      721 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
      781 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
      841 tt
//
