LOCUS Exported 9321 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAG415GPD-EGFP-ccdB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9321) TITLE S cerevisiae Advanced Gateway Destination Vectors REFERENCE 2 (bases 1 to 9321) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9321 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 70..573 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 610..714 /gene="bla" /label=AmpR promoter CDS 715..1575 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(933..952) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1746..2334 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2235..2254 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2488..2505 /label=L4440 /note="L4440 vector, forward primer" promoter 2658..2688 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2696..2712 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2701..2723 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2720..2736 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2720..2736 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2755..2775 /label=T3 /note="T3 promoter, forward primer" promoter 2757..2775 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2804..3448 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 3342..3365 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" primer_bind 3454..3470 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 3454..3470 /label=pBluescriptSK /note="For pBluescript vector" CDS 3483..4199 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3483..4199 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3528..3549) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3789..3808) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4136..4157 /label=EGFP-C /note="EGFP, forward primer" protein_bind 4224..4348 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4373..4403 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 4457..5116 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(4523..4542) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 5461..5766 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 5645..5664 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(5807..5931) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 5938..6185 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(5954..5972) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind complement(6203..6222) /label=T7 /note="T7 promoter, forward primer" promoter complement(6204..6222) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6232..6249) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6232..6248) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6241..6263) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6393..6848 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6480..6499) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6690..6711 /label=F1ori-F /note="F1 origin, forward primer" promoter 7148..7552 /gene="S. cerevisiae LEU2" /label=LEU2 promoter CDS 7565..8659 /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" primer_bind complement(9152..9171) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9271..9293 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact 2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg 2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 2761 aaccctcact aaagggaaca aaagctggag ctcttcgagt ttatcattat caatactcgc 2821 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa 2881 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac 2941 agaatatata acaccgaagg tgcctgggtg aacaggttta ttcctggcat ccactaaata 3001 taatggagcc cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata 3061 ttgttttctt caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac 3121 aggggcacaa acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga 3181 gtaaatgatg acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt 3241 ctattacctt ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc 3301 cctgaaatta ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa 3361 ttctgtaaat ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta 3421 gttttaaaac accagaactt agtttcgacg gattctagaa ctagtggatc ccccgggaca 3481 aaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 3541 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 3601 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 3661 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 3721 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 3781 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 3841 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 3901 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 3961 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 4021 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 4081 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 4141 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg 4201 gtggaccagg tggtggacat caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa 4261 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact 4321 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact 4381 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg 4441 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca 4501 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca 4561 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt 4621 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat 4681 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt 4741 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca 4801 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc 4861 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag 4921 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa 4981 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt 5041 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg 5101 gcagggcggg gcgtaaacgc cgcgtggatc cggcttacta aaagccagat aacagtatgc 5161 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 5221 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat 5281 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 5341 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 5401 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa 5461 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 5521 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 5581 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 5641 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 5701 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 5761 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat 5821 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 5881 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttcgatgtca 5941 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa 6001 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag 6061 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg 6121 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa 6181 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 6241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 6301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 6361 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc 6421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 6481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 6541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 6601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 6661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 6721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 6781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 6841 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 6901 tcgacggtcg aggagaactt ctagtatatc cacataccta atattattgc cttattaaaa 6961 atggaatccc aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact 7021 tccttgttca tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca 7081 acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac 7141 cgcagttaac tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca 7201 accattattt ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa 7261 attcgatgac tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc 7321 aactgaaaaa ttgggagaaa aaggaaaggt gagaggccgg aaccggcttt tcatatagaa 7381 tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt 7441 aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt 7501 accttttaca tttcagcaat atatatatat atttcaagga tataccattc taatgtctgc 7561 ccctatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat 7621 cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt 7681 cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg tcccacttcc 7741 agatgaggcg ctggaagcct ccaagaaggt tgatgccgtt ttgttaggtg ctgtgggtgg 7801 tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga 7861 acttcaattg tacgccaact taagaccatg taactttgca tccgactctc ttttagactt 7921 atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt 7981 gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag 8041 tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct 8101 acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc 8161 aagattatgg agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt 8221 tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa 8281 tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat 8341 cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac 8401 cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt 8461 tgaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc 8521 tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac 8581 tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt 8641 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat 8701 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag 8761 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatagtaa 8821 aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca 8881 ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa 8941 atcatgaaac tacgattcct aatttgatat tggaggattt tctctaaaaa aaaaaaaata 9001 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct 9061 tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc 9121 ttacgacgta gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 9181 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 9241 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 9301 cgtcatcacc gaaacgcgcg a //