LOCUS       Exported                 902 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  The N-terminal component of the Co-InCre system, expresses the 
            N-Cre-N-gp41-1 fusion that generates functional Cre by protein 
            trans-splicing upon reacting with Co-InCreC .
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-Co-InCreN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(503..520)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    509..733
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(805..822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(805..821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(814..836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgccttcttc tttttcctac agatccttaa ttaataatac gactcactat agggggtcga
       61 cccgccacca tgcccaagaa gaagaggaag gtgacctctg atgaagtcag gaagaacctg
      121 atggacatgt tcagggacag gcaggccttc tctgaacaca cctggaagat gctcctgtct
      181 gtgtgcagat cctgggctgc ctggtgcaag ctgaactgcc tggacctgaa gacccaggtg
      241 cagacccctc agggcatgaa ggagatcagc aacatccagg tgggcgacct ggtgctgagc
      301 aacaccggct acaacgaggt gctgaacgtg ttccccaaga gcaagaagaa gagctacaag
      361 atcaccctgg aggacggcaa ggagatcatc tgcagcgagg agcacctgtt ccccacccag
      421 accggcgaga tgaacatcag cggcggcctg aaggagggca tgtgcctgta cgtgaaggag
      481 tgagtcagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      541 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      601 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      661 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      721 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgagatc cactagttct
      781 agcctcgagg ctagagcggc cgccactggc cgtcgtttta caacgtcgtg actgggaaaa
      841 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      901 ta
//
