LOCUS       Exported                1078 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  3rd generation lentiviral packaging plasmid; Contains Rev; also 
            requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    pRSV-Rev
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L
  TITLE     A third-generation lentivirus vector with a conditional packaging 
            system.
  JOURNAL   J Virol. 1998 Nov . 72(11):8463-71.
  PUBMED    9765382
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     25..49
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             143..493
                     /codon_start=1
                     /gene="rev"
                     /product="human immunodeficiency virus 1 (HIV-1) Rev 
                     protein"
                     /label=Rev
                     /note="enhances nuclear export of unspliced and 
                     incompletely spliced viral mRNAs"
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     primer_bind     complement(577..594)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(577..593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1012..1031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cattggtgtg cacctccaag ctcgagctcg tttagtgaac cgtcagatcg cctggagacg
       61 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tcccctcgaa
      121 gctagtcgat taggcatctc ctatggcagg aagaagcgga gacagcgacg aagacctcct
      181 caaggcagtc agactcatca agtttctcta tcaaagcaac ccacctccca atcccgaggg
      241 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      301 ttcgattagt gaacggatcc ttagcactta tctgggacga tctgcggagc ctgtgcctct
      361 tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg
      421 gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacaatat tggagtcagg
      481 agctaaagaa tagtgctgtt agcttgctca atgccacagc tatagcagta gctgagggga
      541 cagatagggt tatagaagta gtacaagaag cttggcactg gccgtcgttt tacaacgtcg
      601 tgatctgagc ctgggagatc tctggctaac tagggaaccc actgcttaag cctcaataaa
      661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      721 gatcaggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      781 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      841 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      901 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      961 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1021 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcg
//
