LOCUS       Exported                4231 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses Cph8 under a modified PLtetO-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pCph8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      11..97
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      937..1031
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1052..1711)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1626..1645
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1712..1814)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1880..1898
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    1919..1937
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1977..1988
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgactagt gcttggattc tcaccaataa aaaacgcccg
      961 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1021 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     1081 actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa
     1141 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1201 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1261 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1321 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1381 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1441 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1501 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1561 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1621 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1681 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1741 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1801 ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtctaagaaa ccattattat
     1861 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagtc
     1921 cctatcagtg atagagatac tgagcacatc agcaggacgc actgaccgaa ttcattaaag
     1981 aggagaaagg tacccatggc caccaccgta caactcagcg accaatccct ccgtcagcta
     2041 gaaaccctcg ccatccacac cgcccacctg attcagcccc acggtttagt ggtggtcctg
     2101 caggaaccag acctcaccat cagccaaatt agcgccaact gcaccggcat tttagggcga
     2161 tcgccagagg atttgttggg cagaacccta ggggaagtgt ttgatagctt tcagattgat
     2221 cccatccaga gtcgcctaac ggccggacaa atcagcagcc tcaaccccag taaactttgg
     2281 gcgcgggtca tgggggacga ctttgtcatt tttgacgggg tttttcatcg caacagtgac
     2341 ggtttattgg tatgtgaact cgagccagcc tacacttccg ataatctgcc cttcctcggt
     2401 ttttatcaca tggccaacgc tgccctgaat cggttgcgcc aacaagctaa tctacgggat
     2461 ttctacgatg ttattgtcga agaagtccgc cgtatgactg gctttgaccg ggtgatgcta
     2521 taccgctttg atgaaaataa ccacggtgat gtcattgccg aagataaacg ggatgatatg
     2581 gaaccctatt tgggcctgca ctatcccgaa tcggatattc cccaacccgc ccgtcggcta
     2641 tttatccaca accccattcg agtaattccc gatgtttatg gtgtggcggt gcccctgacc
     2701 ccagcggtta accccagcac caaccgagcg gtggatttaa cagaatccat tctgcgcagt
     2761 gcgtaccatt gccacttgac ctatctgaaa aatatggggg taggagcgtc tttaaccatt
     2821 tccctaatta aggacggcca tctctggggg ctcattgcct gccaccatca aacccccaaa
     2881 gtaattccct ttgaactgcg taaagcctgc gaattttttg gtcgggtggt gtttagcaac
     2941 atttccgccc aggaagatac ggaaaccttc gattaccggg tgcagctggc ggagcatgaa
     3001 gcggttttat tggacaaaat gaccacggcg gcggattttg tcgaaggatt aactaatcat
     3061 cccgatcgcc tgttgggatt aacgggctcc cagggggcgg ccatttgctt tggggaaaaa
     3121 ttgattttag taggggaaac cccggacgag aaagcagtgc aatatttact gcaatggttg
     3181 gagaatcggg aagtgcaaga cgttttcttc acctcttccc tctcacaaat ttatcctgat
     3241 gcagtgaatt ttaaatccgt ggccagtggc ttattggcca ttcccattgc ccgtcacaac
     3301 tttttgctct ggtttcgccc tgaagtgttg caaacggtta attggggcgg tgacccaaat
     3361 catgcttacg aagctaccca ggaagacggt aaaatcgagc tccatccccg ccaatccttt
     3421 gacctctgga aagaaattgt ccgactccaa tctttgccct ggcaatcggt ggaaatccaa
     3481 agtgccctgg ccctgaaaaa ggcgatcgtc aacctcattt tgcgccaggc agaagaattg
     3541 catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta
     3601 agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag
     3661 caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt
     3721 gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt
     3781 aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc
     3841 gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg
     3901 gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct gggtcaaagt cagcagcgga
     3961 acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa
     4021 caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc
     4081 acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag
     4141 cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg
     4201 cgggcgcagg gcatgacaaa agaagggtaa t
//
