LOCUS       Exported                 926 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Cre dependent expression of GCaMP6f Ca sensor and physically 
            separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 926)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    48..81
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    132..165
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      181..190
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             196..213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             217..249
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             253..276
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      283..292
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             304..360
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     573..594
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(700..721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag cagcgctgtt
      181 gccaccatgg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
      241 caaatgggtc gggatctgta cgacgatgac gataaggatc tcgccaccat ggtcgactca
      301 tcacgtcgta agtggaataa gacaggtcac gcagtcagag ctataggtcg gctgagctca
      361 ctcgagaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttcaag
      421 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
      481 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccaaa
      541 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      601 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
      661 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      721 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
      781 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      841 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      901 gacttcttca agtccgccat gcccga
//
