LOCUS Exported 14287 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion. ACCESSION . VERSION . KEYWORDS GFP-UBR5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14287) AUTHORS Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B, Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C TITLE TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at damaged chromosomes. JOURNAL Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. Epub 2012 Aug 9. PUBMED 22884692 REFERENCE 2 (bases 1 to 14287) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14287 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 105..484 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 485..688 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 639..659 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 685..709 /label=LNCX /note="Human CMV promoter, forward primer" CDS 813..1529 /codon_start=1 /product="Emerald GFP" /label=EmGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(858..879) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1119..1138) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1466..1487 /label=EGFP-C /note="EGFP, forward primer" protein_bind 1545..1569 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(9984..10008) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 10016..10057 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(10097..10116) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10141..10189 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 10391..10817 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10478..10497) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10686..10707 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(10826..10846) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 10831..11160 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 11011..11146 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11073..11092 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 11208..11255 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 11274..11672 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 11830..11951 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11867..11886) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11921..11940 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(12000..12016) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(12000..12016) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(12013..12035) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 12024..12040 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(12048..12078) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12093..12114 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(12231..12248) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12402..12990) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12482..12501) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13161..14021) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 13784..13803 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(14022..14126) /gene="bla" /label=AmpR promoter primer_bind complement(14201..14220) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag 61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac 121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg 721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct 781 agaggatccc taccggtgat atcctcgaga ccatggtgag caagggcgag gagctgttca 841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc 1021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 1261 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc 1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1501 tcactctcgg catggacgag ctgtacaagg gctcgagccc atcaacaagt ttgtacaaaa 1561 aagcaggctt ccggaccatg acgtccatcc atttcgtggt tcacccgctg ccgggcaccg 1621 aggaccagct caatgacagg ttacgagaag tttctgagaa gctgaacaaa tataatttaa 1681 acagccaccc ccctttgaat gtattggaac aggctactat taaacagtgt gtggtgggac 1741 caaatcatgc tgcctttctt cttgaggatg gtagagtttg caggattggt ttttcagtac 1801 agccagacag attggaattg ggtaaacctg ataataatga tgggtcaaag ttgaacagca 1861 actcgggggc agggaggacg tcaaggcctg gtaggacaag cgactctcca tggtttctct 1921 caggttctga gactctaggc aggctggcag gcaacacctt aggaagccgc tggagttctg 1981 gagtgggtgg aagtggtgga ggatcctctg gtaggtcatc agctggagct cgagattccc 2041 gccggcagac tcgagttatt cggacaggac gggatcgagg gtctgggctt ttgggcagtc 2101 agccccagcc agttattcca gcatctgtca ttccagagga gctgatttca caggcccaag 2161 ttgttttaca aggcaaatcc agaagtgtca ttattcgaga acttcagaga acaaatcttg 2221 atgtgaacct tgctgtaaat aatttactta gccgggatga tgaagatgga gatgatgggg 2281 atgatacagc cagcgaatct tatttgcctg gagaggatct tatgtctctc cttgatgccg 2341 acattcattc tgcccaccca agtgtcatta ttgatgcaga tgccatgttt tctgaagaca 2401 ttagctattt tggttaccct tcttttcgtc gttcatcact ttccaggcta ggctcatctc 2461 gagttctcct tcttccctta gagagagact ctgagctgtt gcgtgaacgt gaatccgttt 2521 tacgtttacg tgaacgaagg tggcttgatg gagcctcatt tgataatgaa aggggttcta 2581 ccagcaagga aggagagcca aacttggata agaagaatac acctgttcaa agtccagtat 2641 ctctaggaga agatttgcag tggtggcctg ataaggatgg aacaaaattc atctgtattg 2701 gggctctgta ttctgaactt ctggctgtca gcagtaaagg agaactttat cagtggaaat 2761 ggagtgaatc tgagccttac agaaatgccc agaatccttc attacatcat ccacgagcaa 2821 catttttggg gttaaccaat gaaaagatag tcctcctgtc tgcaaatagc ataagagcaa 2881 ctgtagctac agaaaataac aaggttgcta catgggtgga tgaaacttta agttctgtgg 2941 cttctaaatt agagcacact gctcagactt actctgaact tcaaggagag cggatagttt 3001 ctttacattg ctgtgccctt tacacctgcg ctcagctgga aaacagttta tattggtggg 3061 gtgtagttcc ttttagtcaa aggaagaaaa tgttagagaa agctagagca aaaaataaaa 3121 agcctaaatc cagtgctggt atttcttcaa tgccgaacat cactgttggt acccaggtat 3181 gcttgagaaa taatcctctt tatcatgctg gagcagttgc attttcaatt agtgctggga 3241 ttcctaaagt tggtgtctta atggagtcag tttggaatat gaatgacagc tgtagatttc 3301 aacttagatc tcctgaaagc ttgaaaaaca tggaaaaagc tagcaaaact actgaagcta 3361 agcctgaaag taagcaggag ccagtgaaaa cagaaatggg tcctccacca tctccagcat 3421 ccacgtgtag tgatgcatcc tcaattgcca gcagtgcatc aatgccatac aaacgacgac 3481 ggtcaacccc tgcaccaaaa gaagaggaaa aggtgaatga agagcagtgg tctcttcggg 3541 aagtggtttt tgtggaagat gtcaagaatg ttcctgttgg caaggtgcta aaagtagatg 3601 gtgcctatgt tgctgtaaaa tttccaggaa cctccagtaa tactaactgt cagaacagct 3661 ctggtccaga tgctgaccct tcttctctcc tgcaggattg taggttactt agaattgatg 3721 aattgcaggt tgtcaaaact ggtggaacac cgaaggttcc cgactgtttc caaaggactc 3781 ctaaaaagct ttgtatacct gaaaaaacag aaatattagc agtgaatgta gattccaaag 3841 gtgttcatgc tgttctgaag actggaaatt gggtgcgata ctgtatcttt gatcttgcta 3901 caggaaaagc agaacaggaa aataattttc ctacaagcag cattgctttc cttggtcaga 3961 atgagaggaa tgtagccatt ttcactgctg gacaggaatc tcccattatt cttcgagatg 4021 gaaatggtac catctaccca atggccaaag attgcatggg aggaataagg gatcccgatt 4081 ggctggatct tccacctatt agtagtcttg gaatgggtgt gcattcttta ataaatcttc 4141 ctgccaattc aacaatcaaa aagaaagctg ctgttatcat catggctgta gagaaacaaa 4201 ccttaatgca acacattctg cgctgtgact atgaggcctg tcgacaatat ctaatgaatc 4261 ttgagcaagc ggttgtttta gagcagaatc tacagatgct gcagacattc atcagccaca 4321 gatgtgatgg aaatcgaaat attttgcatg cttgtgtatc agtttgcttt ccaaccagca 4381 ataaagaaac taaagaagaa gaggaagcgg agcgttctga aagaaataca tttgcagaaa 4441 ggctttctgc tgttgaggcc attgcaaatg caatatcagt tgtttcaagt aatggcccag 4501 gtaatcgggc tggatcatca agtagccgaa gtttgagatt acgggaaatg atgagacgtt 4561 cgttgagagc agctggtttg ggtagacatg aagctggagc ttcatccagt gaccaccagg 4621 atccagtttc accccccata gctcccccta gttgggttcc tgaccctcct gcgatggatc 4681 ctgatggtga cattgatttt atcctggccc ccgctgtggg atctcttacc acagcagcaa 4741 ccggtactgg tcaaggacca agcacctcca ctattccagg tccttccaca gagccatctg 4801 tagtagaatc caaggatcga aaggcgaatg ctcattttat attgaaattg ttatgtgaca 4861 gtgtggttct ccagccctat ctacgagaac ttctttctgc caaggatgca agagggatga 4921 ccccatttat gtcagctgta agtggccgag cttatcctgc tgcaattacc atcttagaaa 4981 ctgctcagaa aattgcaaaa gctgaaatat cctcaagtga aaaagaggaa gatgtattca 5041 tgggaatggt ttgcccatca ggtaccaacc ctgatgactc tcctttatat gttttatgtt 5101 gtaatgacac ttgcagtttt acatggactg gagcagagca cattaaccag gatatttttg 5161 agtgtcgaac ttgtggcttg ctggagtcac tgtgttgttg tacggaatgt gcaagggttt 5221 gtcataaagg tcatgattgc aaactcaaac ggacatcacc aacagcctac tgtgattgtt 5281 gggagaaatg taaatgtaaa actcttattg ctggacagaa atctgctcgt cttgatctac 5341 tttatcgcct gctcactgct actaatctgg ttactctgcc aaacagcagg ggagagcacc 5401 tcttactatt cttagtacag acagtcgcaa ggcagacggt ggagcattgt caatacaggc 5461 cacctcgaat cagggaagat cgtaaccgaa aaacagccag tcctgaagat tcagatatgc 5521 cagatcatga tttagagcct ccaagatttg cccagcttgc attggagcgt gttctacagg 5581 actggaatgc cttgaaatct atgattatgt ttgggtcgca ggagaataaa gaccctctta 5641 gtgccagcag tagaataggc catcttttgc cagaagagca agtatacctc aatcagcaaa 5701 gtggcacaat tcggctggac tgtttcactc attgccttat agttaagtgt acagcagata 5761 ttttgctttt agatactcta ctaggtacac tagtgaaaga actccaaaac aaatatacac 5821 ctggacgtag agaagaagct attgctgtga caatgaggtt tctacgttca gtggcaagag 5881 tttttgttat tctgagtgtg gaaatggctt catccaaaaa gaaaaacaac tttattccac 5941 agccaattgg aaaatgcaag cgtgtattcc aagcattgct accttacgct gtggaagaat 6001 tgtgcaacgt agcagagtca ctgattgttc ctgtcagaat ggggattgct cgtccaactg 6061 caccatttac cctggctagt actagcatag atgccatgca gggcagtgaa gaattatttt 6121 cagtggaacc actaccacca cgaccatcat ctgatcagtc tagcagctcc agtcagtctc 6181 agtcatccta catcatcagg aatccacagc agaggcgcat cagccagtca cagcccgttc 6241 ggggcagaga tgaagaacag gatgatattg tttcagcaga tgtggaagag gttgaggtgg 6301 tggagggtgt ggctggagaa gaggatcatc atgatgaaca ggaagaacac ggggaagaaa 6361 atgctgaggc agagggacaa catgatgagc atgatgaaga cgggagtgat atggagctgg 6421 acttgttagc agcagctgaa acagaaagtg atagtgaaag taaccacagc aaccaagata 6481 atgctagtgg gcgcagaagc gttgtcactg cagcaactgc tggttcagaa gcaggagcaa 6541 gcagtgttcc tgccttcttt tctgaagatg attctcaatc gaatgactca agtgattctg 6601 atagcagtag tagtcagagt gacgacatag aacaggagac ctttatgctt gatgagccat 6661 tagaaagaac cacaaatagc tcccatgcca atggtgctgc ccaagctccc cgttcaatgc 6721 agtgggctgt ccgcaacacc cagcatcagc gagcagccag tacagcccct tccagtacat 6781 ctacaccagc agcaagttca gcgggtttga tttatattga tccttcaaac ttacgccgga 6841 gtggtaccat cagtacaagt gctgcagctg cagcagctgc tttggaagct agcaacgcca 6901 gcagttacct aacatctgca agcagtttag ccagggctta cagcattgtc attagacaaa 6961 tctcggactt gatgggcctt attcctaagt ataatcacct agtatactct cagattccag 7021 cagctgtgaa attgacttac caagatgcag taaacttaca gaactatgta gaagaaaagc 7081 ttattcccac ttggaactgg atggtcagta ttatggattc tactgaagct caattacgtt 7141 atggttctgc attagcatct gctggtgatc ctggacatcc aaatcatcct cttcacgctt 7201 ctcagaattc agcgagaaga gagaggatga ctgcgcgaga agaagctagc ttacgaacac 7261 ttgaaggcag acgacgtgcc accttgctta gcgcccgtca aggaatgatg tctgcacgag 7321 gagacttcct aaattatgct ctgtctctaa tgcggtctca taatgatgag cattctgatg 7381 ttcttccagt tttggatgtt tgctcattga agcatgtggc atatgttttt caagcactta 7441 tatactggat taaggcaatg aatcagcaga caacattgga tacacctcaa ctagaacgca 7501 aaaggacgcg agaactcttg gaactgggta ttgataatga agattcagaa catgaaaatg 7561 atgatgacac caatcaaagt gctactttga atgataagga tgatgactct cttcctgcag 7621 aaactggcca aaaccatcca tttttccgac gttcagactc catgacattc cttgggtgta 7681 tacccccaaa tccatttgaa gtgcctctgg ctgaagccat ccccttggct gatcagccac 7741 atctgttgca gccaaatgct agaaaggagg atctttttgg ccgtccaagt cagggtcttt 7801 attcttcatc tgccagtagt gggaaatgtt taatggaggt tacagtggat agaaactgcc 7861 tagaggttct tccaacaaaa atgtcttatg ctgccaatct gaaaaatgta atgaacatgc 7921 aaaaccggca aaaaaaagaa ggggaagaac agcccgtgct gccagaagaa actgagagtt 7981 caaaaccagg gccatctgct catgatcttg ctgcacaatt aaaaagtagc ttactagcag 8041 aaataggact tactgaaagt gaagggccac ctctcacatc tttcaggcca cagtgtagct 8101 ttatgggaat ggttatttcc catgatatgc tgctaggacg ttggcgcctt tctttagaac 8161 tgttcggcag ggtattcatg gaagatgttg gagcagaacc tggatcaatc ctaactgaat 8221 tgggtggttt tgaggtaaaa gaatcaaaat tccgcagaga aatggaaaaa ctgagaaacc 8281 agcagtcaag agatttgtca ctagaggttg atcgggatcg agatcttctc attcagcaga 8341 ctatgaggca gcttaacaat cactttggtc gaagatgtgc tactacacca atggctgtac 8401 acagagtaaa agtcacattt aaggatgagc caggagaggg cagtggtgta gcacgaagtt 8461 tttatacagc cattgcacaa gcatttttat caaatgaaaa attgccaaat ctagagtgta 8521 tccaaaatgc caacaaaggc acccacacaa gtttaatgca gagattaagg aaccgaggag 8581 agagagaccg ggaaagggag agagaaaggg aaatgaggag gagtagtggt ttgcgagcag 8641 gttctcggag ggaccgggat agagacttta gaagacagct ttccatcgac actaggccct 8701 ttagaccagc ctctgaaggg aatcctagcg atgatcctga gcctttgcca gcacatcggc 8761 aggcacttgg agagaggctt tatcctcgtg tacaagcaat gcaaccagca tttgcaagta 8821 aaatcactgg catgttgttg gaattatccc cagctcagct gcttctcctt ctagcaagtg 8881 aggattctct gagagcaaga gtggatgagg ccatggaact cattattgca catggacggg 8941 aaaatggagc tgatagtatc ctggatcttg gattagtaga ctcctcagaa aaggtacagc 9001 aggaaaaccg aaagcgccat ggctctagtc gaagtgtagt agatatggat ttagatgata 9061 cagatgatgg tgatgacaat gcccctttgt tttaccaacc tgggaaaaga ggattttata 9121 ctccaaggcc tggcaagaac acagaagcaa ggttgaattg tttcagaaac attggcagga 9181 ttcttggact atgtctgtta cagaatgaac tctgtcctat cacattgaat agacatgtaa 9241 ttaaagtatt gcttggtaga aaagtcaatt ggcatgattt tgcttttttt gatcctgtaa 9301 tgtatgagag tttgcggcaa ctaatcctcg cgtctcagag ttcagatgct gatgctgttt 9361 tctcagcaat ggatttggca tttgcaattg acctgtgtaa agaagaaggt ggaggacagg 9421 ttgaactcat tcctaatggt gtaaatatac cagtcactcc acagaatgta tatgagtatg 9481 tgcggaaata cgcagaacac agaatgttgg tagttgcaga acagccctta catgcaatga 9541 ggaaaggtct actagatgtg cttccaaaaa attcattaga agatttaacg gcagaagatt 9601 ttaggctttt ggtaaatggc tgcggtgaag tcaatgtgca aatgctgatc agttttacct 9661 ctttcaatga tgaatcagga gaaaatgctg agaagcttct gcagttcaag cgttggttct 9721 ggtcaatagt agagaagatg agcatgacag aacgacaaga tcttgtttac ttttggacat 9781 caagcccatc actgccagcc agtgaagaag gattccagcc tatgccctca atcacaataa 9841 gaccaccaga tgaccaacat cttcctactg caaatacttg catttctcga ctttacgtcc 9901 cactctattc ctctaaacag attctcaaac agaaattgtt actcgccatt aagaccaaga 9961 attttggttt tgtgtagccg cggacccagc tttcttgtac aaagtggttg ataacgggaa 10021 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta 10081 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 10141 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg 10201 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac 10261 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 10321 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg 10381 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 10441 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 10501 tttctcgcca cgttcgccgg ctttcccgtc aagctctaaa tcgggggctc cctttagggt 10561 tccgatttag tgctttacgg cacctcgacc ccaaaaactt gattagggtg atggttcacg 10621 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 10681 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 10741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 10801 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 10861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 10921 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 10981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 11041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 11101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 11161 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat 11221 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca 11281 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca 11341 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct 11401 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg 11461 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga 11521 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg 11581 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat 11641 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga 11701 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 11761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 11821 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 11881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 11941 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 12001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 12061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 12121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 12181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 12241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 12301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 12361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 12421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 12481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 12541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 12601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 12661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 12721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 12781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 12841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 12901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 12961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 13021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 13081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 13141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 13201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 13261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 13321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 13381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 13441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 13501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 13561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 13621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 13681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 13741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 13801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 13861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 13921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 13981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 14041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 14101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac 14161 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg 14221 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga 14281 gcaaaat //