LOCUS       Exported                 953 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Vector for generating dsDNA donors for homology-directed repair. 
            Contains the visible marker 3xP3-DsRed..
ACCESSION   .
VERSION     .
KEYWORDS    pHD-DsRed
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 953)
  TITLE     pHD-DsRed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(79..112)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      338..347
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(474..492)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(524..544)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     742..761
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttatggggtg tcgcccttcg ctgaagcagg tggaattctt gcatgctagc ggccgcggac
       61 atatgcacac ctgcgatcat aacttcgtat aatgtatgct atacgaagtt atcgtacggg
      121 atctaattca attagagact aattcaatta gagctaattc aattaggatc caagcttatc
      181 gatttcgaac cctcgaccgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat
      241 tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg
      301 cagctgaaca agctaaacaa tcggctcgaa gccggtcgcc accatggcct cctccgagga
      361 cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
      421 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      481 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca
      541 gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc
      601 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      661 cgtgacccag gactcctccc tccaggacgg ctccttcatc tacaaggtga agttcatcgg
      721 cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct gggaggcgtc
      781 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggccctgaa
      841 gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc
      901 cgtgcagctg cccggctact actacgtgga ctccaagctg gacatcacct ccc
//
