LOCUS Exported 1029 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.. ACCESSION . VERSION . KEYWORDS pBS-Hsp70-Cas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1029) TITLE FlyCRISPR JOURNAL Unpublished REFERENCE 2 (bases 1 to 1029) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1029 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 18..36 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 64..80 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 65..81 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(115..131) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(115..131) /label=pBluescriptSK /note="For pBluescript vector" promoter 155..607 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" primer_bind 554..575 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" CDS 626..691 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 698..718 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt accgggcccc 61 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca 121 ctagttctag agcggccgcg gaattcccat ccccctagaa tcccaaaaca aactggttat 181 tgtggtaggt catttgtttg gcagaaagaa aactcgagaa atttctctgg ccgttattcg 241 ttattctctc ttttcttttt gggtctctcc ctctctgcac taatgctctc tcactctgtc 301 acacagtaaa cggcatactg ctctcgttgg ttcgagagag cgcgcctcga atgttcgcga 361 aaagagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac 421 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa 481 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc 541 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag 601 aactctgggg gatctgatcg atatggacta taaggaccac gacggagact acaaggatca 661 tgatattgat tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg 721 tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa 781 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt 841 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga 901 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag 961 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga 1021 cgacagctt //