LOCUS Exported 12044 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor. ACCESSION . VERSION . KEYWORDS pMDC32B-AtMIR390a-B/c SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12044) AUTHORS Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC TITLE New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. JOURNAL Plant Physiol. 2014 Mar 19. PUBMED 24647477 REFERENCE 2 (bases 1 to 12044) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12044 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 425..1219 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 1306..1894 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1795..1814 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2048..2065 /label=L4440 /note="L4440 vector, forward primer" misc_feature 2080..2220 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(2146..2165) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2306..2328 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 2564..2758 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(2824..3891) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" CDS complement(4325..4954) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature 6254..6278 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 6466..6488 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6480..6497 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6481..6497 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 6924..7268 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 7212..7232 /label=35S promoter /note="CaMV 35S promoter, forward primer" protein_bind 7328..7352 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7646..7676 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 7730..8389 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(7796..7815) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 8731..9036 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 8915..8934 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(9300..9324) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(9653..9669) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9653..9669) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9666..9688) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9677..9693 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9701..9731) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9746..9767 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9958..10635 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" CDS 10703..11728 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" polyA_signal 11768..11942 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature 12020..12044 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc 61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc 121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga 181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc 241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg 301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg 361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata 421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg 481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga 541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg 601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt 661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc 721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat 781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg 841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg 901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa 961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa 1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta 1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga 1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt 1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc 1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct 2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga 2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg 2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg 2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc 2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt 2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg 2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc 2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat 2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg 2761 aaagagaact tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt 2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat 2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat 2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta 3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta 3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc 3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta 3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc 3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt 3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg 3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc 3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag 3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc 3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac 3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa 3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg 3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg 3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag 3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg 3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta 3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt 4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat 4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg 4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg 4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt 4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat 4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt 4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca 4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa 4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc 4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata 4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca 4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg 4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt 4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat 4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc 4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt 4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca 5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc 5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc 5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa 5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc 5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc 5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga 5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg 5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg 5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg 5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac 5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt 5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg 5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc 5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc 5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg 5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca 6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac 6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg 6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca 6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc 6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa 6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc 6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac 6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag 6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt 6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat 6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca 6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca 6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc 6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt 7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc 7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt 7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac 7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg 7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag 7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca cctatagggg 7381 ggaaaaaaag gtagtcatca gatatatatt ttggtaagaa aatatagaaa tgaataattt 7441 cacgtttaac gaagaggaga tgacgtgtgt tccttcgaac ccgagttttg ttcgtctata 7501 aatagcacct tctcttctcc ttcttcctca cttccatctt tttagcttca ctatctctct 7561 ataatcggtt ttatctttct ctaagtcaca acccaaaaaa acaaagtaga gaagaatctg 7621 taagagacca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 7681 gattttgagt taggagccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa 7741 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 7801 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 7861 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 7921 ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat 7981 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 8041 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 8101 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 8161 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 8221 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 8281 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat 8341 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggagc 8401 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag 8461 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt 8521 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata 8581 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct 8641 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct 8701 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga 8761 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccggccga 8821 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 8881 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 8941 gtgccggttt ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 9001 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc 9061 cagtctgcac ctcgacggtc tcacattggc tcttcttact acaatgaaaa aggccgaggc 9121 aaaacgccta aaatcacttg agaatcaatt ctttttactg tccatttaag ctatctttta 9181 taaacgtgtc ttattttcta tctcttttgt ttaaactaag aaactatagt attttgtcta 9241 aaacaaaaca tgaaagaaca gattagatct catctttagt ctcaagggtg ggcgcgccga 9301 cccagctttc ttgtacaaag tggttcgata attccttaat taactagttc tagagcggcc 9361 gcccaccgcg gtggagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct 9421 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg 9481 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga 9541 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact 9601 aggataaatt atcgcgcgcg gtgtcatcta tgttactgaa ttcgtaatca tggtcatagc 9661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 9721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 9781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 9841 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga 9901 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga 9961 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg 10021 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga 10081 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc 10141 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga 10201 ttgatgtgat aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac 10261 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct 10321 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg 10381 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc 10441 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt 10501 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga 10561 cgcacaatcc cactatcctt cgcaagacct tcctctatat aaggaagttc atttcatttg 10621 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc 10681 agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa 10741 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga 10801 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg 10861 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc 10921 gattccggaa gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg 10981 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca 11041 accggtcgcg gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 11101 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 11161 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 11221 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 11281 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 11341 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 11401 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 11461 tccggagctt gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca 11521 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 11581 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 11641 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 11701 cagcactcgt ccgagggcaa agaaatagag tagatgccga ccggatctgt cgatcgacaa 11761 gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta 11821 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa 11881 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga 11941 tcccccgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa 12001 gttgtctaag cgtcaatttg tttacaccac aatatatcct gcca //