LOCUS Exported 3728 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol. ACCESSION . VERSION . KEYWORDS pGEM-and3 in situ probe SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3728) TITLE actinodin in situ probes JOURNAL Unpublished REFERENCE 2 (bases 1 to 3728) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3728 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 33..42 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 37..46 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(853..871) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(854..871) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(889..905) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(889..905) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(902..924) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 913..929 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(937..967) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 982..1003 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1120..1137) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1291..1879) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1371..1390) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2050..2910) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2673..2692 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2911..3015) /gene="bla" /label=AmpR promoter rep_origin complement(3093..3548) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3230..3251) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3442..3461 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3674..3696 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3688..3705 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3689..3705 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3712..3 /label=T7 /note="T7 promoter, forward primer" promoter 3712..2 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 61 tcgtcagagg agatctgtgg atcccaactg gtatcgcaaa aatcccgact ttcagtccta 121 ctatcgttac tataatagta ttgggcacac agaagggctc tatgagatcg ataaaattag 181 aatgctttac cagcaaatgc gtcatctgga gctcacctat ggccctgacg catcgaaata 241 ccagaatacc cttggtctaa agccgacccc tccacccaca actcagcctc caccccctac 301 tttaccacct gtctccaaga tgaatgttat atacctgtgc aattcaaaag acccccagtg 361 caagccacat attgtctacc tgcccgccgg tggtgtgcct gtactttgcg atccacgtta 421 ccatccaaac tgtaaactaa gtcctgccca gaattctccc tcaccacctg taattgtccc 481 agaaccagtg acgactgtcc ctcttccacc aacacaaaaa tccactccac caccacctcc 541 tcctcctata atggttgtca aaggaatgga gtatgactgt gatccttact gggaccctga 601 ctgcctgatc gataatcctc caaggccagt aaaaatggtt gaaccgtcat cagagctccc 661 agatgagaag gtgaaaaagg aaattccagt cgatccagtg ccaacctatg acccatatga 721 cttcaatcaa gacctgtatg acccctttcg ccatgctgct ccagctcact aaaatcacta 781 gtgaattcgc ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca 841 tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt 901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 1021 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 1081 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 1141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 1201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 1261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 1321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 1381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 1441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 1501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 1561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 1621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 1681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 1741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 1801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1981 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 2041 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 2101 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 2161 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 2221 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 2281 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 2341 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 2401 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 2461 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 2521 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 2581 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 2641 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 2701 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 2761 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 2821 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2881 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2941 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 3001 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc 3061 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa 3121 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3181 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3241 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3301 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3361 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3421 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 3481 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 3541 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 3601 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 3661 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac 3721 tcactata //