LOCUS       Exported                 905 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  p2attNG landing pad plasmid with 400 bp homology arms to the human 
            H11 locus (for use with TALEN-assisted recombination)..
ACCESSION   .
VERSION     .
KEYWORDS    p2attNG-H11-short
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    535..634
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        804..905
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagctgga gctgatttaa atgactcaga
       61 actgtactgt attctgtaga atctttatcc gtaagtgcta tgtatatact aatagttaag
      121 gtgaataata aaacaattgt gagctaagga atgtgataca gaacacaagg agtcaattaa
      181 tgcatgagtc ttaagggaaa ttcttagact tcagtgggtc taagctcaga actctgagct
      241 tagagaaaca ggatgcccca gtaaacatga tttgtttgag agaaactacc atatgtaata
      301 gatgattcct caataaactg ttttcactag gtcagtttaa tcttggaaat tacactatag
      361 atatcctggt attagccaca aggctatgct atctataggg tcagtccagt ataaactgac
      421 ctgttggggt ctttaaagct caaggaaaaa ggagatcttt tctcgactag tactgacgga
      481 cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg
      541 tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg
      601 ggggcgtagg gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg
      661 tgcttacgac cgtcagtcgc gcgagcgcga ctagtcgaga aggatccttt gtcgacgaag
      721 ttcctattcc gaagttccta ttcttcaaaa ggtataggaa cttcgctagc gattattcag
      781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      901 ggaag
//
