LOCUS Exported 316 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION Encodes module 1 with amino acids 12S-16R for recognition of nt 8C. ACCESSION . VERSION . KEYWORDS pChlr-1SR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 316) AUTHORS Abil Z, Denard CA, Zhao H TITLE Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. JOURNAL J Biol Eng. 2014 Mar 1;8(1):7. PUBMED 24581042 REFERENCE 2 (bases 1 to 316) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..316 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(121..137) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(121..137) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(134..156) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 145..161 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(169..199) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 214..235 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 tttcccaaga ccagcatggg tccagattca ttcgcctgaa actggagcgt gccacaccag 61 ctgagcgcca gcttgtcttc aatgaaatcc tccagagaga ccaagcttgg cgtaatcatg 121 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 181 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 241 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 301 cggccaacgc gcgggg //