LOCUS Exported 785 bp ds-DNA linear SYN 06-JUN-2021 DEFINITION Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor. ACCESSION . VERSION . KEYWORDS pMDC123SB-AtMIR390a-B/c SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 785) AUTHORS Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC TITLE New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. JOURNAL Plant Physiol. 2014 Mar 19. PUBMED 24647477 REFERENCE 2 (bases 1 to 785) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..785 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 302..607 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 486..505 /label=ccdB-fwd /note="ccdB gene, forward primer" ORIGIN 1 cgtatttgcg cgctgatttt tgcggnataa gaatatatac tgatatgtat acccgaagta 61 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta 121 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag 181 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct 241 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga 301 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 361 acagagtgat attattgaca cgcccggccg acggatggtg atccccctgg ccagtgcacg 421 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 481 ctggcgcatg atgaccaccg atatggccag tgtgccggtt tccgttatcg gggaagaagt 541 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 601 aatataaatg tcaggctccc ttatacacag ccagtctgca cctcgacggt ctcacattgg 661 ctcttcttac tacaatgaaa aaggccgagg caaaacgcct aaaatcactt gagaatcaat 721 tctttttact gtccatttaa gctatctttt ataaacgtgt cttattttct atctcttttg 781 tttaa //