LOCUS       Exported                 863 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Gateway-compatible entry vector for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor .
ACCESSION   .
VERSION     .
KEYWORDS    pENTR-AtTAS1c-B/c
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(659..758)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(775..794)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(776..794)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(799..815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(799..815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca cctcgacggt ctcagaacta
      121 gaaaagacat tggacatatt ccaggatatg caaaagaaaa caatgaatat tgttttgaat
      181 gtgttcaagt aaatgagatt ttcaagtcgt ctaaagaaca gttgctaata cagttactta
      241 tttcaataaa taattggttc taataataca aaacatattc gaggatatgc agaaaaaaag
      301 atgtttgtta ttttgaaaag cttgagtagt ttctctccga ggtgtagcga agaagcatca
      361 tctactttgt aatgtaattt tctttatgtt ttcactttgt aattttattt gtgttaatgt
      421 accatggccg atatcggttt tattgaaaga aaatttatgt tacttctgtt ttggctttgc
      481 aatcagttat gctagttttc ttataccctt tcgtaagctt cctaaggaat cgttcattga
      541 tttccactgc ttcattgtat attaaaactt tacaactgta tcgaccatca tataattctg
      601 ggtcaagaga tgaaaataga acaccacatc gtaaagtgaa ataagggtgg gcgcgccgac
      661 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
      721 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      781 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
      841 ctctgatgtt acattgcaca aga
//
