LOCUS Exported 789 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Lentiviral plasmid expressing the reverse tetracycline transactivator. ACCESSION . VERSION . KEYWORDS FUW-M2rtTA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 789) AUTHORS Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R TITLE A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):346-53. PUBMED 18786421 REFERENCE 2 (bases 1 to 789) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..789 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 79..90 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 488..508 /label=WPRE-R /note="WPRE, reverse primer" CDS complement(607..717) /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" ORIGIN 1 gactcgtctg agggcgaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg 61 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag 121 aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac 181 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc 241 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca 301 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc 361 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa 421 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc 481 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat 541 tttgtaatcc agaggttgat tatcgataag cttgatatcg aattgatcct tacttagtta 601 cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc 661 aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggc 721 cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc 781 gaaaaggaa //