LOCUS Exported 237 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION Encodes module 6 with amino acids 12S-16R for recognition of nt 3C. ACCESSION . VERSION . KEYWORDS pChlr-6SR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 237) AUTHORS Abil Z, Denard CA, Zhao H TITLE Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. JOURNAL J Biol Eng. 2014 Mar 1;8(1):7. PUBMED 24581042 REFERENCE 2 (bases 1 to 237) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..237 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(144..160) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(144..160) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(157..179) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 168..184 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(192..222) /label=lac promoter /note="promoter for the E. coli lac operon" ORIGIN 1 gtctcacaca cagagcagct tgtacaggat caatatggaa gttatgtaat ccgccatgta 61 ctggagcacg gtcgtcctga ggataaaagc aaaattgtag cagaaatccg aggcaatgag 121 agaccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 181 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaa //