LOCUS       Exported                6879 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses full-length Leucine rich repeat containing 59 ectodomain 
            in mammalian cells. N-terminal His tag, biotinylation sequence and 
            rat Cd4d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    LRRC59-His-bio
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      1..10
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    979..1034
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1033..1052)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3270..3288)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3356..3460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3461..4321
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3679..3698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4492..5080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4981..5000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5234..5251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5368..5389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5404..5434
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5442..5458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5447..5469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5466..5482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5466..5482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5753..6132
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6133..6336
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6287..6307
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6333..6357
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6838..6857
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgccttgca gagtgaccga gctgcagcag cagcccctgt gcaccagcgt gaacaccatc
      841 tacgacaacg ccgtgcaggg cctgcggcgg cacgagattc tgcagtgggt gctgcagacc
      901 gacagccagc agtgaggcgc gcctgaattc ttctagacac gtgattaatt aaggatcccc
      961 cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     1021 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc
     1081 ggagatctct agctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     1141 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     1201 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     1261 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     1321 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     1381 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     1441 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     1501 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     1561 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     1621 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     1681 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     1741 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     1801 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     1861 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     1921 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     1981 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     2041 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     2101 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     2161 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     2221 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     2281 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     2341 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     2401 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     2461 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     2521 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     2581 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     2641 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     2701 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     2761 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     2821 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     2881 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
     2941 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     3001 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     3061 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     3121 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     3181 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca
     3241 aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3301 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3361 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3421 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3481 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3541 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3601 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3661 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     3721 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3781 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3841 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3901 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3961 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     4021 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4081 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4141 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4201 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4261 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4321 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4381 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4441 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4501 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4561 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4621 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4681 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4741 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4801 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4861 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4921 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4981 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5041 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5101 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5161 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5221 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5281 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5341 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5401 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5461 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt
     5521 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     5581 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     5641 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     5701 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     5761 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5821 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5881 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5941 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6001 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6061 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     6121 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     6181 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6241 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     6301 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcctc
     6361 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac
     6421 tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtactcc
     6481 gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc
     6541 gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg
     6601 gtcggggttg tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag
     6661 acggcggatg gtcgaggtga ggtgtggcag gcttgagatc cagctgttgg ggtgagtact
     6721 ccctctcaaa agcgggcatt acttctgcgc taagattgtc agtttccaaa aacgaggagg
     6781 atttgatatt cacctggccc gatctggcca tacacttgag tgacaatgac atccactttg
     6841 cctttctctc cacaggtgtc cactcccagg tccaagttt
//
