LOCUS Exported 610 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Expression of a GFP-dependent transcription factor component in mammalian cells. ACCESSION . VERSION . KEYWORDS pCAG-GBP1-10gly-lexADBD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 610) AUTHORS Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska B, Cepko CL TITLE A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. JOURNAL Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021. PUBMED 23953120 REFERENCE 2 (bases 1 to 610) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..610 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 26..48 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 110..129 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 194..203 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 209..550 /codon_start=1 /product="GFP-binding fragment of a single-chain camelid antibody (Rothbauer et al., 2008)" /label=GFP nanobody /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYSNVNVGFEYW GQGTQVTVSS" ORIGIN 1 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga 61 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg 121 gttattgtgc tgtctcatca ttttggcaaa gaattctgca gtcgacggta ccgcgggccc 181 gggatccacc ggtgccacca tggccgacgt gcagctcgtg gaatccggag gagcactggt 241 ccagcctggg ggatcactga gactgtcttg tgccgcttct ggctttcctg tgaatcggta 301 ctctatgaga tggtacaggc aggcacctgg aaaagagcga gaatgggtcg ccggaatgtc 361 atctgctggc gataggagtt cctacgagga tagcgtgaaa ggacggttca ccattagtag 421 ggacgatgct agaaacaccg tgtacctcca gatgaactcc ctgaaacctg aggacactgc 481 tgtgtactac tccaacgtca atgtgggctt tgaatactgg ggacagggca cacaagtgac 541 tgttagttct gctagcggag gaggtggtgg aggtggaggt ggaggtacta gtatgaaagc 601 gttaacggcc //