LOCUS Exported 15606 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses TagRFP-T and presynaptic mGRASP component. ACCESSION . VERSION . KEYWORDS pRVdG-4RFP-5premGRASP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15606) TITLE Genetic Neuroengineering Group Plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 15606) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15606 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 3..459 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(90..109) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 300..321 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 585..607 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 599..616 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 658..1037 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1038..1241 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1192..1212 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 1286..1305 /label=T7 /note="T7 promoter, forward primer" promoter 1286..1304 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 4695..4704 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4701..5432 /codon_start=1 /product="photostable variant of TagRFP (Shaner et al., 2008)" /label=TagRFP-T /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV ARYCDLPSKLGHKLNGMDELYK" CDS 5604..5651 /codon_start=1 /product="11th beta-strand of superfolder GFP" /label=GFP11 /note="associates with GFP(1-10), consisting of the first 10 beta-strands, to reconstitute fluorescent GFP (Cabantous et al., 2005; Kamiyama et al., 2016)" /translation="RDHMVLHEYVNAAGIT" CDS 8267..8278 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(13278..13296) /label=T7 Term /note="T7 terminator, reverse primer" terminator 13292..13339 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(13362..13378) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13362..13378) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(13415..13435) /label=T3 /note="T3 promoter, forward primer" promoter complement(13415..13433) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(13454..13470) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13454..13470) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13467..13489) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13478..13494 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13502..13532) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13547..13568 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13685..13702) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13856..14444) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13936..13955) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14615..15475) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 15238..15257 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(15476..15580) /gene="bla" /label=AmpR promoter ORIGIN 1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat 421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac 661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac 1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct 1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca 1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca 1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag 1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt 1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg 1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt 1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga 1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg 1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta 1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac 1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa 1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata 2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta 2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta 2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc 2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct 2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat 2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt 2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt 2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc 2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca 2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga 2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac 2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag 2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat 2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca 2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag 2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc 3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat 3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag 3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat 3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca 3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag 3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc 3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct 3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct 3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc 3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa 3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta 3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa 3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg 3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa 3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag 3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg 3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa 4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct 4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt 4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg 4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga 4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg 4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa 4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga 4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca 4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata 4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac 4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg 4681 ggcccgggaa agccgccacc atggtgtcta agggcgaaga gctgattaag gagaacatgc 4741 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg 4801 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc 4861 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc agaaccttca 4921 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat 4981 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc 5041 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg 5101 gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg ctgtaccccg 5161 ctgacggcgg cctggaaggc agaaccgaca tggccctgaa gctcgtgggc gggggccacc 5221 tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc 5281 ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac aaagagacct 5341 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc 5401 acaaacttaa tggcatggac gagctgtaca agtaatgatt aattaacgtc ctttcaacga 5461 tccaagtcca tgaaaaaaac taacacccct cccgtacgaa agccgccacc atgccacctt 5521 ctactagtct tttgttgctt gctgcacttc tccccttcgc tcttcccgct tcagattgga 5581 agacaggaga agtcaccgct tcccgcgacc acatggtgct gcacgaatat gtgaacgctg 5641 ctggcatcac aggcggtggc ggatctggcg gcggtggtag cgtcgacttt cagaaagcta 5701 gctctattgt ttataagaag gagggggaac aggtggagtt cagtttccca ttggccttca 5761 cagtcgagaa gctcacagga tcaggagagt tgtggtggca ggccgaaaga gcctcctcca 5821 gcaagagttg gatcactttt gatctgaaaa acaaagaagt gagcgtgaag agagtcacac 5881 aggaccctaa gctgcagatg ggcaagaagt tgcctttgca cttgacactc ccccaagccc 5941 tgccccagta tgctggctcc gggaacctca ccctcgcctt ggaggctaag accggaaagt 6001 tgcatcagga ggtcaacctc gtggtgatga gggcaacaca gctgcagaag aacctgacct 6061 gcgaagtgtg gggtcccacc tcacctaaac tgatgctgtc tctgaagctg gagaataagg 6121 aggctaaagt cagtaaaaga gagaaggctg tgtgggtcct taacccggag gcagggatgt 6181 ggcagtgtct tcttagcgac tctggacagg tgctgctgga gagcaacatc aaggtgcttc 6241 ctacttggtc aacaccagta cagccgatgg ccctgatcgt actcggggga gtcgccggcc 6301 tgcttctgtt tatcggcctg ggcatcttct tctgcgtacg ctacaagtat cgcaacaggg 6361 atgagggatc ttaccacgtt gacgaatcta ggaactatat cagtaacagc gctcagagta 6421 atggcgccgt ggttaaagag aaacagccct ccagcgccaa atcagcaaac aaaaacaaga 6481 aaaacaagga taaagagtac tacgtaagat cttaatgagc tagcttataa agtgctgggt 6541 catctaagct tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc 6601 tcaacctgag acttacttca agatgctcga tcctggagag gtctatgatg accctattga 6661 cccaatcgag ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa 6721 ctctgactac aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg 6781 gttaaaaaca gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt 6841 cagagttttg aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat 6901 ggctgcacag tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag 6961 ccggagatgt ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct 7021 gttgaatctc acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg 7081 ccttgagagg gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc 7141 ttacttgttc ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa 7201 gaccatccta gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa 7261 gttcaaagac caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag 7321 ttccaattgt ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg 7381 cttcaactcc ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat 7441 atctcaacta tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc 7501 cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc 7561 agaaaagttt aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa 7621 ggtaagtcaa cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga 7681 tcaattcgac aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca 7741 cccatatata gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa 7801 aatgatagat aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag 7861 atggggtttt gataagtact ccaagtggta tctggattca agattcctag cccgagacca 7921 ccccttgact ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt 7981 gggggataca tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga 8041 tccgtcagaa atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg 8101 gctgtcagaa aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc 8161 taagccgcct gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga 8221 tgaagacttg ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt 8281 ctttgctcta atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc 8341 caactacatc ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt 8401 taaaaagctg atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata 8461 tgcatttcac ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga 8521 tgtattttct gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga 8581 gttttttcaa aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga 8641 ggatcaaata tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg 8701 cgggctagaa ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag 8761 agaatctcaa atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt 8821 atgtccgaca tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga 8881 gagaatatca aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct 8941 agggctgatc atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa 9001 aacccctttg tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc 9061 ttgcgtctct aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa 9121 tgcgctaaca gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct 9181 catgtcagta caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt 9241 ttacaagatt ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta 9301 tctagatcct tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca 9361 gttctcagac cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca 9421 agagtcctgg attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac 9481 actcgagagc ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc 9541 cagtcctacc attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa 9601 ggtggaaaat tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt 9661 tatactcttc ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag 9721 ttcgtctttt ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat 9781 aagaaggcag tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga 9841 gatccacggg attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg 9901 ctcttcagag agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac 9961 gacagttcct cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac 10021 ttgtggagca acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga 10081 tcagtcattt ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc 10141 gacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct 10201 atcgttaaaa gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct 10261 aattaggaac attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt 10321 caaaaggacg gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta 10381 ttcttctgtc tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga 10441 tttgacccaa gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca 10501 gacatggaca tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg 10561 gcacctccga tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca 10621 gatcttcgag tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc 10681 tcacttccag aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg 10741 tagagaaaag tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc 10801 aattcacgac tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt 10861 ttcccctaga gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg 10921 cttcttgaca agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt 10981 aatctcatat attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga 11041 accgtctctt agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac 11101 cactatgaaa gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga 11161 gcgagagata atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag 11221 aagtgccaaa atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag 11281 ggttgagaga aacctatcta agagtatgag agataacctg cgacaattga gttctttgat 11341 gaggcaggtg ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg 11401 actgctaaaa gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc 11461 tagaaccatg actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc 11521 agaatgggtc tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt 11581 ctcggagctt gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt 11641 gagagtggtt cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct 11701 caatgttttc ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc 11761 agtcctcaac atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga 11821 cctgatggct tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga 11881 tatcgtctcc agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa 11941 cttggcaacc tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct 12001 cattatttgc gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat 12061 gtccgatttt gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac 12121 tatgctagta aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt 12181 cacagggttt atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc 12241 caaacgaggg aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat 12301 gagccttgtg ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt 12361 gaactatcag gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga 12421 gatgatcata actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga 12481 tgatcttgag ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat 12541 agttttctcc aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc 12601 accgtctgat cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct 12661 atctactgct ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc 12721 tataacttat tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg 12781 gtccaacgac acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc 12841 tcactggatc aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa 12901 ggatctatcc agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga 12961 ggatatcaga tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg 13021 gaagcctgcc catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat 13081 ctgaaccttt ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgtggg 13141 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgcacgt 13201 ccactcggat ggctaaggga ggggcgggga tccggctgct aacaaagccc gaaaggaagc 13261 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg 13321 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatccacta gttctagagc 13381 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct 13441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 13501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 13561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 13621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 13681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 13741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 13801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 13861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 13921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 13981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 14041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 14101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 14161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 14221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 14281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 14341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 14401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 14461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 14521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 14581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 14641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 14701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 14761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 14821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 14881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 14941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 15001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 15061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 15121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 15181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 15241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 15301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 15361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 15421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 15481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 15541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 15601 cacctg //