LOCUS Exported 816 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION 3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter. ACCESSION . VERSION . KEYWORDS pTet-O-Ngn2-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 816) AUTHORS Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, Sudhof TC TITLE Rapid single-step induction of functional neurons from human pluripotent stem cells. JOURNAL Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029. PUBMED 23764284 REFERENCE 2 (bases 1 to 816) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..816 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(63..662) /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(146..166) /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind 643..662 /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" CDS complement(669..722) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" ORIGIN 1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattagc tagcttaatt 61 aatcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg 121 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc 181 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg 241 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg 301 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg 361 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg 421 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg 481 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg 541 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg 601 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc 661 atctcgagtg ggccgggatt ttcctccacg tccccgcatg ttagaagact tcccctgccc 721 tctctagaga tacagtccct ggcgaggggc aggtgaggcg cataacgatg cttctccgga 781 ggtgggggct gccagtagtc cacgtctgac ccgggg //